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BrightLine® Sedat laser filter set, optimized for 405, 488, & 594 nm laser sources

Semrock Set / Fluorophore Graphing

Part Number: LF405/488/594-3X3M-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Blue
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
1,8-ANS3724771-Star
4-methylumbelliferone3574411-Star
7-hydroxy-4-methylcoumarin3604423-Star
Alexa Fluor® 4054014221-Star
AMCA (Aminomethylcoumarin)3504483-Star
BD Horizon V4504064493-Star
BFP (EBFP)3804405-Star
Calcofluor White3514441-Star
Cascade Blue™4014191-Star
CellTrace calcein violet4004523-Star
DAPI3594615-Star
DiFMU-pH 9.03584501-Star
DyLight 4053984203-Star
ER-Tracker™ Blue-White DPX3725571-Star
HiLyte Fluor™ 4054034283-Star
Hoechst 332583524551-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034553-Star
LysoSensor Blue3744241-Star
LysoTracker Blue3734221-Star
Marina Blue3634611-Star
Pacific Blue™4044553-Star
sgBFP™3874513-Star
Sirius3554243-Star
SpectrumBlue4054493-Star
TagBFP4024571-Star
Vybrant DyeCycle Violet3694373-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005253-Star
5-FAM (5-carboxyfluorescein)4925183-Star
6-carboxyrhodamine 6G5255551-Star
Acridine Orange (+DNA)5025263-Star
Alexa Fluor® 4304315411-Star
Alexa Fluor® 4884995203-Star
Alexa Fluor® 5005035253-Star
AmCyan14584893-Star
ATTO 4885005253-Star
ATTO 4954955273-Star
BCECF (pH 5.5)4815183-Star
BCECF (pH 9.0)5025283-Star
BODIPY FL5055123-Star
BODIPY FL-MeOH5025113-Star
Calcein4945143-Star
Calcium Green-15075291-Star
CFP24905103-Star
Cy2™4925073-Star
CyQUANT GR-DNA5025233-Star
DAF-FM-NO4955193-Star
Dendra2 (Green)4915073-Star
DiA4575861-Star
DiO4755003-Star
Dronpa5025163-Star
DY-505-Phalloidin5035303-Star
ecliptic pHluorin pH5.54735073-Star
Emerald4915113-Star
evoglow-Bs14494961-Star
FITC (Fluorescein)4955195-Star
FlAsH-CCPFCC5115301-Star
Fluo-35065271-Star
Fluo-44945163-Star
Fluorescein dextran5015243-Star
Fluorescein-pH 8.04895173-Star
Fluoro-Emerald4945183-Star
FluoSpheres Yellow-Green fluorescent microspheres5035143-Star
FM 1-434735791-Star
GFP (EGFP)4895115-Star
Green 4964965203-Star
Green 5005015243-Star
HCS LipidTOX Green neutral lipid stain4985073-Star
HCS LipidTOX Green phospholipidosis5045363-Star
HiLyte Fluor™ 4884975263-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoSensor Green4485023-Star
LysoTracker Green5015093-Star
LysoTracker Yellow HCK-1234885651-Star
Magnesium Green5075311-Star
mHoneyDew4785621-Star
MitoTracker™ Green4905123-Star
mWasabi4935093-Star
NBD-X (MeOH)4675383-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955243-Star
Oregon Green™ 4884985263-Star
Oregon Green™ 5145135321-Star
PicoGreen®5025223-Star
PKH674885003-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005431-Star
ratiometric pHluorin pH55005093-Star
Rhodamine 1104975193-Star
Rhodamine 1235075291-Star
Rhodamine Green4975233-Star
Rhodol Green4975243-Star
sgGFP™ (super glow GFP)4725063-Star
Sodium Green5075323-Star
SpectrumGreen4975383-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985223-Star
SYBR Safe DNA gel stain-DNA5095261-Star
SYTO 115065251-Star
SYTO 134485063-Star
SYTO 164895203-Star
SYTO 94835003-Star
SYTO RNASelect geen fluorescent cell stain5035273-Star
SYTOX Green-DNA5045243-Star
TO-PRO-15155311-Star
TOTO-15145311-Star
TurboGFP4825033-Star
Vybrant DyeCycle Green5065343-Star
wtGFP (wild type GFP, non-UV excitation)4745093-Star
YO-PRO-14915063-Star
YOYO-14915083-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxynapthofluorescein (pH 10)5556153-Star
5-ROX (carboxy-X-rhodamine)5786041-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Alexa Fluor® 5945906183-Star
APC6516601-Star
APC (Allophycocyanin)6516601-Star
ATTO 5905946243-Star
ATTO 5946016243-Star
ATTO 6106146341-Star
ATTO 6206186421-Star
ATTO 6356356581-Star
ATTO 6476446701-Star
BODIPY 630/650-X6256411-Star
BODIPY TR-X phallacidin5906213-Star
Calcium Crimson5896091-Star
Carboxynaphthofluorescein5996743-Star
CellTrace BODIPY TR methyl ester5986253-Star
CellTracker Red CMTPX5866143-Star
ChlorophyllA4806503-Star
DDAO6486573-Star
DyLight 5945936183-Star
DyLight 6336206491-Star
FluoSpheres Crimson fluorescent microspheres6206463-Star
FluoSpheres Red fluorescent microspheres5776071-Star
HcRed15886183-Star
HCS CellMask Red cytoplasmic6226441-Star
HCS LipidTOX Deep Red neutral lipid stain6356521-Star
HCS LipidTOX Red neutral lipid stain5826163-Star
HCS LipidTOX Red phospholipidosis5846081-Star
HiLyte Fluor™ 5945926163-Star
Hypericin6006031-Star
KFP-Red5755991-Star
LIVE-DEAD Fixable Red Dead Cell Stain5956133-Star
mCherry5876105-Star
MitoTracker™ Deep Red6416621-Star
mKate25886333-Star
mPlum5896493-Star
mRFP15847001-Star
Nile blue-EtOH6316601-Star
Nile red-phospolipid5536373-Star
Qdot® 625 Nanocrystals3006213-Star
Qdot® 655 Nanocrystals3006543-Star
ReAsH-CCPGCC5926061-Star
Red 5805806031-Star
SNARF (carboxy) 514 Excitation pH95766383-Star
SNARF-1 488nm (ph 9.0)5766383-Star
SNARF-1 514nm (ph 9.0)5766383-Star
SpectrumRed5876153-Star
SYTO 176196381-Star
SYTO 596226441-Star
SYTO 616186503-Star
Texas Red dextran5926133-Star
Texas Red DHPE5846081-Star
Texas Red®5926143-Star
TOTO-36426611-Star
TurboFP635(Katushka)5916383-Star
YO-PRO-36136293-Star
YOYO-36116313-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.
Revised Filter Set

LF405_488_594-3X3M-B.png

BrightLine® Sedat laser filter set, optimized for 405, 488, & 594 nm laser sources

Laser-optimized 7-filter "Sedat" triple-band set with 3 exciters, 3 emitters and a multiband beamsplitter. Ideal for use with: Blue: DAPI; Green: FITC , GFP ; Red: mCherry, Texas Red
Part # LF405/488/594-3X3M-B-000
$2195
(Non-US pricing info)


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US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.



LF405/488/594-3X3M-A

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