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BrightLine® single-band filter set, optimized for Sirius and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: Sirius-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Single Band ExciterFF01-370/36
Single Band EmitterFF01-440/40
Single Band DichroicCFW-Di01-Clin

General Filter Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
7-amino-4-methylcoumarin (AMC)3464421-Star
Alexa Fluor® 3503434411-Star
Alexa Fluor® 4054014221-Star
AMCA (Aminomethylcoumarin)3504483-Star
BD Horizon V4504064491-Star
BFP (EBFP)3804403-Star
Calcofluor White3514443-Star
Cascade Blue™4014191-Star
CellTrace calcein violet4004521-Star
DiFMU-pH 9.03584503-Star
DyLight 3503514343-Star
FluoSpheres Blue fluorescent microspheres3564143-Star
HiLyte Fluor™ 4054034281-Star
Hoechst 332583524553-Star
LIVE-DEAD Fixable Blue Dead Cell Stain3434411-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034551-Star
LysoSensor Blue3744241-Star
LysoTracker Blue3734223-Star
Marina Blue3634613-Star
Pacific Blue™4044551-Star
Quinine sulfate3494613-Star
Vybrant DyeCycle Violet3694373-Star

Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
White Paper
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.

Sirius-A Sirius

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