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BrightLine® long-pass filter set, optimized for SYBR Gold and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: SYBRGold/LP-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Single Band ExciterFF01-497/16
Long Pass EmitterFF01-519/LP
Single Band DichroicFF516-Di01

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005251-Star
6-carboxyrhodamine 6G5255551-Star
6-JOE5205481-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Acridine Orange (+DNA)5025261-Star
Acridine Orange (+RNA)4606501-Star
Alexa Fluor® 4884995201-Star
Alexa Fluor® 5005035251-Star
Alexa Fluor® 5145185431-Star
ATTO 4885005251-Star
ATTO 4954955271-Star
ATTO 5205165381-Star
BCECF (pH 5.5)4815181-Star
BCECF (pH 9.0)5025283-Star
BODIPY R6G5285471-Star
BODIPY TMR-X5445731-Star
BODIPY TR-X (MeOH)5445731-Star
Calcium Green-15075293-Star
CyQUANT GR-DNA5025231-Star
Dendra2 (Red)5545691-Star
Di-8-ANEPPS4676311-Star
DiA4575861-Star
DsRed-Express5565841-Star
DY-505-Phalloidin5035303-Star
Eosin5255461-Star
Ethidium bromide5186033-Star
Ethidium homodimer5276171-Star
FITC (Fluorescein)4955191-Star
FlAsH-CCPFCC5115303-Star
Fluo-35065271-Star
Fluorescein dextran5015241-Star
Fluoro-Emerald4945181-Star
FM 1-434735791-Star
Gold 5255215511-Star
Green 4964965201-Star
Green 5005015241-Star
HCS LipidTOX Green phospholipidosis5045363-Star
HiLyte Fluor™ 4884975261-Star
JC-14995251-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985251-Star
LysoTracker Yellow HCK-1234885653-Star
Magnesium Green5075313-Star
mHoneyDew4785623-Star
mRFP15847001-Star
NBD-X (MeOH)4675381-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955241-Star
Nile red-phospolipid5536371-Star
Nile red-triglyceride5105833-Star
OFP5475671-Star
Oregon Green™ 4884985261-Star
Oregon Green™ 5145135321-Star
PicoGreen®5025221-Star
POPO-35335741-Star
Propidium Iodide (PI)3056171-Star
Qdot® 565 Nanocrystals3005641-Star
Qdot® 585 Nanocrystals3005881-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 655 Nanocrystals3006541-Star
Rhodamine 1235075293-Star
Rhodamine Green4975231-Star
Rhodol Green4975241-Star
R-phycoerythrin (PE)5655761-Star
SNARF-1 488nm (ph 6.0)5485861-Star
SNARF-1 514nm (ph 6.0)5495871-Star
Sodium Green5075323-Star
SpectrumGreen4975381-Star
SYBR Gold nucleic acid gel stain-DNA4965395-Star
SYBR Green I nucleic acid gel stain-DNA4985221-Star
SYBR Safe DNA gel stain-DNA5095261-Star
SYPRO Ruby protein gel stain4696311-Star
SYTO 115065251-Star
SYTO 164895201-Star
SYTO RNASelect geen fluorescent cell stain5035271-Star
SYTOX Green-DNA5045241-Star
TO-PRO-15155311-Star
TOTO-15145311-Star
TurboYFP5255381-Star
Venus5165281-Star
VIC5305501-Star
Vybrant DyeCycle Green5065343-Star
Vybrant DyeCycle Orange5195631-Star
YFP (yellow GFP)5135301-Star
ZsYellow15295391-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



SYBRGold/LP-A SYBRGold

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