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BrightLine® Sedat laser filter set, optimized for 488 & 561 nm laser sources

Semrock Set / Fluorophore Graphing

Part Number: LF488/561-2X2M-B-000

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Semrock Filter Set: LF488/561-2X2M-B

BrightLine® Sedat laser filter set, optimized for 488 & 561 nm laser sources

This "Sedat" multiband set includes single-band exciter and emission filters, and a dual-band dichroic. It is optimized for laser excitation utilizing a 473 or 488 nm laser source for GFP or FITC and a 559, 561.4 or 568.2 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF488/561-2X2M-C


Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details LF488/561-2X2M-B-000 (Standard size set, fits most microscopes2)$1,555Ships in 2 daysAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF02-482/18-25$305
Single Band Exciter25 mm x 5.0 mmFF01-563/9-25$305
Single Band Emitter25 mm x 3.5 mmFF01-525/45-25$305
Single Band Emitter25 mm x 3.5 mmFF01-609/54-25$305
Dual Band Dichroic25.2 mm x 35.6 mm x 1.1 mmDi01-R488/561-25x36$455
Click to expand/collapse details LF488/561-2X2M-B-L01 (Leica 'Large' set)$1,555Ships in 2 daysAdd To Cart
Click to expand/collapse details LF488/561-2X2M-B-S01 (Sutter threaded set)$1,635Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details LF488/561-2X2M-B-NQF (set mounted in cube)$1,950Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details LF488/561-2X2M-B-NTE (set mounted in cube)$1,950Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details LF488/561-2X2M-B-OMF (set mounted in cube)$1,990Ships in 2 daysAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details LF488/561-2X2M-B-OFF (set mounted in cube)$1,990Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details LF488/561-2X2M-B-ZHE (set mounted in cube)$1,830Ships in 2 daysAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details LF488/561-2X2M-B-LDMK (set mounted in cube)$1,905Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005253-Star
5-FAM (5-carboxyfluorescein)4925183-Star
6-carboxyrhodamine 6G5255551-Star
Acridine Orange (+DNA)5025263-Star
Alexa Fluor® 4304315411-Star
Alexa Fluor® 4884995203-Star
Alexa Fluor® 5005035253-Star
AmCyan14584893-Star
ATTO 4885005253-Star
ATTO 4954955273-Star
BCECF (pH 5.5)4815183-Star
BCECF (pH 9.0)5025283-Star
BODIPY FL5055123-Star
BODIPY FL-MeOH5025113-Star
Calcein4945143-Star
Calcium Green-15075291-Star
CFP24905103-Star
Cy2™4925073-Star
CyQUANT GR-DNA5025233-Star
DAF-FM-NO4955193-Star
Dendra2 (Green)4915073-Star
DiA4575861-Star
DiO4755003-Star
Dronpa5025163-Star
DY-505-Phalloidin5035303-Star
ecliptic pHluorin pH5.54735073-Star
Emerald4915113-Star
evoglow-Bs14494961-Star
FITC (Fluorescein)4955195-Star
FlAsH-CCPFCC5115301-Star
Fluo-35065271-Star
Fluo-44945163-Star
Fluorescein dextran5015243-Star
Fluorescein-pH 8.04895173-Star
Fluoro-Emerald4945183-Star
FluoSpheres Yellow-Green fluorescent microspheres5035143-Star
FM 1-434735791-Star
GFP (EGFP)4895115-Star
Green 4964965203-Star
Green 5005015243-Star
HCS LipidTOX Green neutral lipid stain4985073-Star
HCS LipidTOX Green phospholipidosis5045363-Star
HiLyte Fluor™ 4884975263-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoSensor Green4485023-Star
LysoTracker Green5015093-Star
LysoTracker Yellow HCK-1234885651-Star
Magnesium Green5075311-Star
mHoneyDew4785621-Star
MitoTracker™ Green4905123-Star
mWasabi4935093-Star
NBD-X (MeOH)4675383-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955243-Star
Oregon Green™ 4884985263-Star
Oregon Green™ 5145135321-Star
PicoGreen®5025223-Star
PKH674885003-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005431-Star
ratiometric pHluorin pH55005093-Star
Rhodamine 1104975193-Star
Rhodamine 1235075291-Star
Rhodamine Green4975233-Star
Rhodol Green4975243-Star
sgGFP™ (super glow GFP)4725063-Star
Sodium Green5075323-Star
SpectrumGreen4975383-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985223-Star
SYBR Safe DNA gel stain-DNA5095261-Star
SYTO 115065251-Star
SYTO 134485063-Star
SYTO 164895203-Star
SYTO 94835003-Star
SYTO RNASelect geen fluorescent cell stain5035273-Star
SYTOX Green-DNA5045243-Star
TO-PRO-15155311-Star
TOTO-15145311-Star
TurboGFP4825033-Star
Vybrant DyeCycle Green5065343-Star
wtGFP (wild type GFP, non-UV excitation)4745093-Star
YO-PRO-14915063-Star
YOYO-14915083-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Orange
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825953-Star
5-ROX (carboxy-X-rhodamine)5786043-Star
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)5425683-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Alexa Fluor® 5465565721-Star
Alexa Fluor® 5685796033-Star
Alexa Fluor® 5945906181-Star
Amplex UltraRed peroxidation product (pH 7.5)5685813-Star
AsRed 25765923-Star
ATTO 5505535763-Star
ATTO 5655635923-Star
ATTO 5905946241-Star
BOBO™-35706053-Star
BODIPY TMR-X5445731-Star
BODIPY TR-X (MeOH)5445731-Star
BODIPY TR-X phallacidin5906213-Star
Calcium Crimson5896091-Star
Calcium Orange5896091-Star
CellTrace BODIPY TR methyl ester5986251-Star
CellTracker Red CMTPX5866143-Star
Cy3.5™5785913-Star
Dendra2 (Red)5545691-Star
DsRed5595833-Star
DsRed-Express5565843-Star
dTomato5565823-Star
DY-5905825993-Star
DyLight 5945936181-Star
Ethidium bromide5186031-Star
Ethidium homodimer5276173-Star
FluoSpheres Red fluorescent microspheres5776073-Star
HcRed15886181-Star
HCS LipidTOX Red neutral lipid stain5826163-Star
HCS LipidTOX Red phospholipidosis5846083-Star
HiLyte Fluor™ 5945926161-Star
Hypericin6006031-Star
KFP-Red5755993-Star
LOLO-15685803-Star
LysoTracker Red5735923-Star
Magnesium Orange5505741-Star
mApple5665943-Star
mCherry5876105-Star
Merocyanine 5405595793-Star
MitoTracker™ Orange5515751-Star
MitoTracker™ Red5785983-Star
mKate25886333-Star
mPlum5896491-Star
mRFP5555833-Star
mRFP15847005-Star
mRuby5586053-Star
mStrawberry5745963-Star
mTangerine5685853-Star
Nile red-phospolipid5536373-Star
Orange 5525525741-Star
pHrodo™, succinimidyl ester5605873-Star
Propidium Iodide (PI)3056173-Star
Pro-Q Diamond5565833-Star
Qdot® 585 Nanocrystals3005883-Star
Qdot® 605 Nanocrystals3006023-Star
Qdot® 625 Nanocrystals3006211-Star
ReAsH-CCPGCC5926063-Star
Red 5805806033-Star
Resorufin5715843-Star
Rhod-25535773-Star
Rhodamine phalloidin5585751-Star
Rhodamine Red-X5735913-Star
R-phycoerythrin (PE)5655763-Star
SNARF (carboxy) 514 Excitation pH95766381-Star
SNARF-1 488nm (ph 6.0)5485863-Star
SNARF-1 488nm (ph 9.0)5766381-Star
SNARF-1 514nm (ph 6.0)5495873-Star
SNARF-1 514nm (ph 9.0)5766381-Star
SpectrumOrange5545873-Star
Sulforhodamine 101-EtOH5785933-Star
SYPRO Ruby protein gel stain4696311-Star
Tetramethylrhodamine dextran5545823-Star
Texas Red dextran5926131-Star
Texas Red DHPE5846083-Star
TRITC (Tetramethylrhodamine)5525783-Star
TurboFP635(Katushka)5916381-Star
TurboRFP5535731-Star
X-Rhod-1 Indicator5806013-Star
YOYO-36116311-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.
Revised Filter Set

LF488_561-2X2M-C.png

BrightLine® Sedat 1λ P-V RWE super-resolution laser filter set, optimized for 405, 488, 561, & 635 nm laser sources

1λ P-V RWE super-resolution laser optimized 9-filter "Sedat" quad-band set with 4 exciters, 4 emitters and a multiband beamsplitter. Ideal for use with: Blue: DAPI; Green: FITC , GFP ; Orange: mRFP1, mCherry; Red: Cy5
Part # LF488/561-2X2M-C-000
$1595
(Non-US pricing info)


[x]
US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.



LF488/561-2X2M-B 488/561 Sedat Dual-Band Laser-Set Di01-R488/561 FF02-482/18 FF01-609/54 FF01-563/9 FF01-525/45 R488/561 488/561 482/18 609/54 563/9 525/45 Filter Filters Set Sets Dual 2X2M LF488/561-2X2M-B-000 LF488/561-2X2M-B-L01 LF488/561-2X2M-B-S01 LF488/561-2X2M-B-NQF LF488/561-2X2M-B-NTE LF488/561-2X2M-B-OMF LF488/561-2X2M-B-OFF LF488/561-2X2M-B-ZHE LF488/561-2X2M-B-LDMK

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