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BrightLine® long-pass laser filter set, optimized for 405 nm laser sources

Semrock Set / Fluorophore Graphing

Part Number: LF405/LP-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
1,8-ANS3724773-Star
4-methylumbelliferone3574411-Star
7-hydroxy-4-methylcoumarin3604423-Star
Alexa Fluor® 3503434413-Star
Alexa Fluor® 4054014225-Star
Alexa Fluor® 4304315413-Star
AMCA (Aminomethylcoumarin)3504483-Star
Aqua 4314304781-Star
ATTO 3903924773-Star
ATTO 4254364841-Star
BD Horizon V4504064493-Star
BD Horizon V5004105003-Star
BFP (EBFP)3804403-Star
Calcofluor White3514441-Star
Cascade Blue™4014193-Star
Cascade Yellow™3995493-Star
CellTrace calcein violet4004523-Star
CellTracker Violet BMQC+GSH4065263-Star
CFP (ECFP)4334751-Star
ChlorophyllA4806503-Star
DAPI3594615-Star
Dapoxyl3735793-Star
DEAC4324723-Star
DiFMU-pH 9.03584501-Star
DY-4154184703-Star
DyLight 4053984203-Star
ER-Tracker™ Blue-White DPX3725573-Star
Fura Red™ (high pH)5726573-Star
Fura-2 (calcium free)3665153-Star
GFPuv3955093-Star
HiLyte Fluor™ 4054034283-Star
Hoechst 332583524551-Star
Hoechst 333423524553-Star
Hoechst 345803924403-Star
LIVE-DEAD Fixable Aqua Dead Cell Stain3675263-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034553-Star
Lucifer yellow4285443-Star
LysoSensor Blue3744243-Star
LysoTracker Blue3734223-Star
Marina Blue3634613-Star
mBBr+GSH3944903-Star
Pacific Blue™4044553-Star
Pacific Orange4005513-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005433-Star
Qdot® 565 Nanocrystals3005643-Star
Qdot® 585 Nanocrystals3005883-Star
Qdot® 605 Nanocrystals3006023-Star
Qdot® 625 Nanocrystals3006213-Star
Qdot® 655 Nanocrystals3006543-Star
Qdot® 705 Nanocrystals3007023-Star
Qdot® 800 Nanocrystals3007923-Star
sgBFP™3874513-Star
Sirius3554243-Star
SpectrumAqua4344811-Star
SpectrumBlue4054493-Star
SYPRO Ruby protein gel stain4696313-Star
TagBFP4024573-Star
ThiolTracker Violet+GSH4045263-Star
Vybrant DyeCycle Violet3694373-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.
Revised Filter Set

LF405_LP-B.png

BrightLine® long-pass laser filter set, optimized for 405 nm laser sources

Laser optimized long-pass filter set.
Ideal for use with: DAPI, Hoechst 33258, Hoechst 33342, Hoechst 34580, Alexa Fluor 350, Alexa Fluor 405, ACMA , BFP
Part # LF405/LP-B-000
$915
(Non-US pricing info)


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US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.



LF405/LP-A

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