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BrightLine® single-band laser filter set, optimized for 635 nm laser sources

Semrock Set / Fluorophore Graphing

Part Number: LF635-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxynapthofluorescein (pH 10)5556151-Star
Alexa Fluor® 6336316475-Star
Alexa Fluor® 6356336481-Star
Alexa Fluor® 6476536685-Star
Alexa Fluor® 6606636913-Star
APC6516603-Star
APC (Allophycocyanin)6516605-Star
ATTO 6336296583-Star
ATTO 6356356583-Star
ATTO 6476446703-Star
ATTO 647N6456683-Star
ATTO 6556636833-Star
BODIPY 650/665-X6466645-Star
Carboxynaphthofluorescein5996741-Star
Cy5.5™6726901-Star
Cy5™6496665-Star
DDAO6486573-Star
DiD (DiIC18(5)) - Lipophilic Tracer6486705-Star
DY-6306346563-Star
DyLight 6336206491-Star
DyLight 6496516733-Star
FluoSpheres Crimson fluorescent microspheres6206461-Star
FluoSpheres Dark Red fluorescent microspheres6566833-Star
HCS CellMask Red cytoplasmic6226441-Star
HCS LipidTOX Deep Red neutral lipid stain6356521-Star
HiLyte Fluor™ 6476496733-Star
LIVE-DEAD Fixable Far Red Dead Cell Stain6536683-Star
MitoTracker™ Deep Red6416623-Star
Nile blue-EtOH6316603-Star
Qdot® 705 Nanocrystals3007021-Star
Red 6506496653-Star
SpectrumFRed (Far Red)6506733-Star
SYTO 596226441-Star
SYTO 606506813-Star
SYTO 616186501-Star
SYTOX Red-DNA6406583-Star
TO-PRO-36426573-Star
TOTO-36426613-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.
Revised Filter Set

LF635-B.png

BrightLine® single-band laser filter set, optimized for 635 nm laser sources

Laser optimized single-band filter set. Ideal for use with: Cy5, Alexa Fluor 633, Alexa Fluor 647, APC , BODIPY 650/665-X, DiD ) - Lipophilic Tracer
Part # LF635-B-000
$915
(Non-US pricing info)


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US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.



LF635-A

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