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BrightLine® Pinkel filter set, optimized for Blue & Green Fluorescent Proteins & HcRed and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: BFP/GFP/HcRed-3X-A-000

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Semrock Filter Set: BFP/GFP/HcRed-3X-A

BrightLine® Pinkel filter set, optimized for Blue & Green Fluorescent Proteins & HcRed and other like fluorophores

This filter set is ideal for imaging samples that are triple-labeled with blue, green, and red fluorescent proteins (BFP, GFP, and HcRed in particular). It is also excellent for samples with a DAPI (or Hoechst) stain in the blue channel, and other green or red fluorophores like FITC or Texas Red in the green and red channels, respectively. The red band is very wide to allow maximum brightness in this channel.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details BFP/GFP/HcRed-3X-A-000 (Standard size set, fits most microscopes2)$1,565In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-370/36-25$305
Single Band Exciter25 mm x 5.0 mmFF01-474/23-25$405
Single Band Exciter25 mm x 5.0 mmFF01-585/29-25$305
Triple Band Emitter25 mm x 3.5 mmFF01-425/527/685-25$465
Triple Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF395/495/610-Di01-25x36$465
Click to expand/collapse details BFP/GFP/HcRed-3X-A-L01 (Leica 'Large' set)$1,565Ships in 2 daysAdd To Cart
Click to expand/collapse details BFP/GFP/HcRed-3X-A-S01 (Sutter threaded set)$1,625Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details BFP/GFP/HcRed-3X-A-NQF (set mounted in cube)$1,960In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details BFP/GFP/HcRed-3X-A-NTE (set mounted in cube)$1,960In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details BFP/GFP/HcRed-3X-A-OMF (set mounted in cube)$2,000In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details BFP/GFP/HcRed-3X-A-OFF (set mounted in cube)$2,000In StockAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details BFP/GFP/HcRed-3X-A-ZHE (set mounted in cube)$1,840In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details BFP/GFP/HcRed-3X-A-LDMK (set mounted in cube)$1,915Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details BFP/GFP/HcRed-3X-A-LDMP (set mounted in cube)$2,000In StockAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Blue
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
4-methylumbelliferone3574413-Star
7-amino-4-methylcoumarin (AMC)3464421-Star
7-hydroxy-4-methylcoumarin3604421-Star
Alexa Fluor® 3503434415-Star
Alexa Fluor® 4054014221-Star
AMCA (Aminomethylcoumarin)3504485-Star
BD Horizon V4504064491-Star
BFP (EBFP)3804405-Star
Calcofluor White3514443-Star
Cascade Blue™4014191-Star
DAPI3594615-Star
DiFMU-pH 9.03584501-Star
DyLight 3503514343-Star
FluoSpheres Blue fluorescent microspheres3564143-Star
HiLyte Fluor™ 4054034281-Star
Hoechst 332583524551-Star
Hoechst 333423524553-Star
Hoechst 345803924403-Star
LIVE-DEAD Fixable Blue Dead Cell Stain3434411-Star
LysoSensor Blue3744241-Star
LysoTracker Blue3734223-Star
Marina Blue3634611-Star
Quinine sulfate3494611-Star
sgBFP™3874511-Star
Sirius3554243-Star
Vybrant DyeCycle Violet3694373-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-FAM (5-carboxyfluorescein)4925183-Star
Acridine Orange (+DNA)5025261-Star
Acridine Yellow4705501-Star
Alexa Fluor® 4304315411-Star
Alexa Fluor® 4884995205-Star
Alexa Fluor® 5005035251-Star
AmCyan14584891-Star
ATTO 4654535071-Star
ATTO 4885005251-Star
ATTO 4954955271-Star
BCECF (pH 5.5)4815183-Star
BCECF (pH 9.0)5025281-Star
BOBO™-14614841-Star
BODIPY FL5055121-Star
BODIPY FL-MeOH5025111-Star
Calcein4945141-Star
CFP24905103-Star
Cy2™4925073-Star
CyQUANT GR-DNA5025231-Star
DAF-FM-NO4955191-Star
Dendra2 (Green)4915073-Star
DiA4575861-Star
DiO4755003-Star
Dronpa5025161-Star
ecliptic pHluorin pH5.54735073-Star
Emerald4915113-Star
evoglow-Bs14494961-Star
FITC (Fluorescein)4955195-Star
Fluo-44945161-Star
Fluorescein dextran5015241-Star
Fluorescein-pH 8.04895171-Star
Fluoro-Emerald4945181-Star
FluoSpheres Yellow-Green fluorescent microspheres5035141-Star
FM 1-434735791-Star
GFP (EGFP)4895115-Star
Green 4964965201-Star
Green 5005015241-Star
HCS LipidTOX Green phospholipidosis5045361-Star
HiLyte Fluor™ 4884975261-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985251-Star
LysoSensor Green4485021-Star
LysoTracker Green5015091-Star
LysoTracker Yellow HCK-1234885651-Star
mHoneyDew4785621-Star
MitoTracker™ Green4905123-Star
mTFP14624921-Star
mWasabi4935091-Star
NBD-X (MeOH)4675383-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955241-Star
Oregon Green™ 4884985261-Star
PicoGreen®5025221-Star
PKH674885003-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005431-Star
ratiometric pHluorin pH55005091-Star
Rhodamine 1104975191-Star
Rhodamine Green4975231-Star
Rhodol Green4975241-Star
sgGFP™ (super glow GFP)4725063-Star
Sodium Green5075321-Star
SpectrumGreen4975381-Star
SYBR Gold nucleic acid gel stain-DNA4965391-Star
SYBR Green I nucleic acid gel stain-DNA4985221-Star
SYBR Safe DNA gel stain-DNA5095261-Star
SYTO 115065251-Star
SYTO 134485063-Star
SYTO 164895203-Star
SYTO 94835003-Star
SYTO RNASelect geen fluorescent cell stain5035271-Star
SYTOX Green-DNA5045241-Star
TagCFP4584801-Star
TOTO-15145311-Star
TurboGFP4825033-Star
Vybrant DyeCycle Green5065341-Star
wtGFP (wild type GFP, non-UV excitation)4745093-Star
YO-PRO-14915063-Star
YOYO-14915083-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Yellow
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Alexa Fluor® 5685796033-Star
Cy3.5™5785913-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxynapthofluorescein (pH 10)5556153-Star
5-ROX (carboxy-X-rhodamine)5786041-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Alexa Fluor® 5945906185-Star
Alexa Fluor® 6606636911-Star
APC6516601-Star
APC (Allophycocyanin)6516601-Star
ATTO 5905946243-Star
ATTO 5946016243-Star
ATTO 6106146341-Star
ATTO 6206186421-Star
BOBO™-35706051-Star
BODIPY 630/650-X6256411-Star
BODIPY TR-X phallacidin5906213-Star
Calcium Crimson5896091-Star
Carboxynaphthofluorescein5996743-Star
CellTrace BODIPY TR methyl ester5986253-Star
CellTracker Red CMTPX5866143-Star
ChlorophyllA4806503-Star
DDAO6486573-Star
DyLight 5945936183-Star
DyLight 6336206491-Star
FluoSpheres Crimson fluorescent microspheres6206461-Star
FluoSpheres Red fluorescent microspheres5776071-Star
FM 4-645057251-Star
HcRed15886185-Star
HCS CellMask Red cytoplasmic6226441-Star
HCS LipidTOX Deep Red neutral lipid stain6356521-Star
HCS LipidTOX Red neutral lipid stain5826163-Star
HCS LipidTOX Red phospholipidosis5846081-Star
HiLyte Fluor™ 5945926163-Star
LDS 7515607113-Star
LIVE-DEAD Fixable Red Dead Cell Stain5956131-Star
mApple5665941-Star
mCherry5876101-Star
mKate25886333-Star
mPlum5896493-Star
mRFP15847001-Star
Nile blue-EtOH6316601-Star
Nile red-phospolipid5536371-Star
PerCP4906771-Star
Propidium Iodide (PI)3056171-Star
ReAsH-CCPGCC5926061-Star
Red 5805806031-Star
SNARF (carboxy) 514 Excitation pH95766383-Star
SNARF-1 488nm (ph 9.0)5766383-Star
SNARF-1 514nm (ph 9.0)5766383-Star
SpectrumRed5876153-Star
SYTO 176196381-Star
SYTO 596226441-Star
SYTO 616186501-Star
Texas Red dextran5926133-Star
Texas Red DHPE5846081-Star
Texas Red®5926141-Star
TurboFP635(Katushka)5916383-Star
YO-PRO-36136291-Star
YOYO-36116311-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Journal Article
TopicDescription
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



BFP/GFP/HcRed-3X-A BFP/GFP/HcRed BFP GFP HcRed Pinkel Triple-Band FF395/495/610-Di01 FF01-474/23 FF01-425/527/685 FF01-370/36 FF01-585/29 585/29 370/36 474/23 425/527/685 FF395/495/610 395/495/610 Filter Filters Set Sets Triple 3X BFP/GFP/HcRed-3X-A-000 BFP/GFP/HcRed-3X-A-L01 BFP/GFP/HcRed-3X-A-S01 BFP/GFP/HcRed-3X-A-NQF BFP/GFP/HcRed-3X-A-NTE BFP/GFP/HcRed-3X-A-OMF BFP/GFP/HcRed-3X-A-OFF BFP/GFP/HcRed-3X-A-ZHE BFP/GFP/HcRed-3X-A-LDMP BFP/GFP/HcRed-3X-A-LDMK

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