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BrightLine® full-multiband filter set, optimized for Cyan & Yellow Fluorescent Proteins and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: CFP/YFP-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Cyan
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Alexa Fluor® 4054014221-Star
AmCyan14584893-Star
Aqua 4314304781-Star
ATTO 3903924771-Star
ATTO 4254364841-Star
BD Horizon V4504064493-Star
BD Horizon V5004105001-Star
Cascade Blue™4014191-Star
CellTrace calcein violet4004523-Star
Cerulean4344731-Star
CFP (ECFP)4334755-Star
DEAC4324723-Star
DY-4154184703-Star
ECFP4354753-Star
HiLyte Fluor™ 4054034281-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034553-Star
mBBr+GSH3944901-Star
Pacific Blue™4044553-Star
POPO-14334573-Star
PO-PRO-14334563-Star
SpectrumAqua4344811-Star
SpectrumBlue4054493-Star
SYTOX Blue4444705-Star
TagBFP4024573-Star
TagCFP4584801-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Yellow
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005253-Star
5-FAM (5-carboxyfluorescein)4925181-Star
6-carboxyrhodamine 6G5255551-Star
6-JOE5205481-Star
Acridine Orange (+DNA)5025263-Star
Alexa Fluor® 4884995201-Star
Alexa Fluor® 5005035253-Star
Alexa Fluor® 5145185431-Star
Alexa Fluor® 5325345531-Star
ATTO 4885005253-Star
ATTO 4954955273-Star
ATTO 5205165381-Star
BCECF (pH 5.5)4815181-Star
BCECF (pH 9.0)5025283-Star
BOBO™-14614843-Star
BODIPY R6G5285471-Star
Calcein4945141-Star
Calcium Green-15075295-Star
CyQUANT GR-DNA5025231-Star
DAF-FM-NO4955191-Star
DiA4575861-Star
DiO4755001-Star
Dronpa5025161-Star
DY-505-Phalloidin5035303-Star
Eosin5255465-Star
FITC (Fluorescein)4955191-Star
FlAsH-CCPFCC5115303-Star
Fluo-35065273-Star
Fluo-44945161-Star
Fluorescein dextran5015241-Star
Fluorescein-pH 8.04895171-Star
Fluoro-Emerald4945181-Star
FluoSpheres Orange fluorescent microspheres5405591-Star
FluoSpheres Yellow-Green fluorescent microspheres5035141-Star
FM 1-434735791-Star
Gold 5255215511-Star
Green 4964965201-Star
Green 5005015241-Star
HCS LipidTOX Green phospholipidosis5045363-Star
HiLyte Fluor™ 4884975263-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoTracker Yellow HCK-1234885653-Star
Magnesium Green5075313-Star
mHoneyDew4785623-Star
MitoTracker™ Green4905121-Star
mKO5495591-Star
mOrange5465621-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955241-Star
OFP5475671-Star
Oregon Green™ 4884985263-Star
Oregon Green™ 5145135323-Star
PicoGreen®5025221-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005431-Star
Qdot® 565 Nanocrystals3005641-Star
Rhodamine 1104975191-Star
Rhodamine 1235075295-Star
Rhodamine Green4975231-Star
Rhodol Green4975241-Star
Sodium Green5075323-Star
SpectrumGreen4975381-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985221-Star
SYBR Safe DNA gel stain-DNA5095263-Star
SYTO 115065253-Star
SYTO 134485061-Star
SYTO 164895201-Star
SYTO RNASelect geen fluorescent cell stain5035273-Star
SYTOX Green-DNA5045241-Star
TO-PRO-15155313-Star
TOTO-15145313-Star
TurboYFP5255381-Star
Venus5165285-Star
VIC5305501-Star
Vybrant DyeCycle Green5065343-Star
Vybrant DyeCycle Orange5195631-Star
YFP (yellow GFP)5135305-Star
YO-PRO-14915061-Star
YOYO-14915081-Star
ZsYellow15295391-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Journal Article
TopicDescription
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



CFP/YFP-A

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