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BrightLine® single-band filter set, optimized for Tryptophan and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: TRP-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Semrock Filter Set: TRP-A

BrightLine® single-band filter set, optimized for Tryptophan and other like fluorophores

Unique UV fluorescence filter set! Only Semrock offers true UV fluorescence filters with high transmission, edge steepness, and blocking that rival high-performance visible filters. The filters in this set are ideal for imaging the intrinsic fluorescence from aromatic amino acids (like tryptophan) and other UV-excited fluorescent species. Note: This set is optimized for systems using a light source (such as a UV LED or filtered Xe arc lamp) with no near-infrared (near-IR) intensity and/or a detector (such as a photomultiplier tube or IR-filtered CCD camera) that is not sensitive to near-IR light. It is not designed to block near-IR light – for applications involving a light source with appreciable near-IR intensity and a near-IR-sensitive detector, contact Semrock.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details TRP-A-000 (Standard size set, fits most microscopes2)$1,025In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-280/20-25$405
Single Band Emitter25 mm x 3.5 mmFF01-357/44-25$305
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF310-Di01-25x36$435
Click to expand/collapse details TRP-A-L01 (Leica 'Large' set)$1,025Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details TRP-A-NQF (set mounted in cube)$1,420In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details TRP-A-NTE (set mounted in cube)$1,420In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details TRP-A-OMF (set mounted in cube)$1,460In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details TRP-A-OFF (set mounted in cube)$1,460In StockAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details TRP-A-ZHE (set mounted in cube)$1,300In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details TRP-A-LDMK (set mounted in cube)$1,375Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details TRP-A-LDMP (set mounted in cube)$1,460In StockAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Naphthalene2753223-Star
Purine2724053-Star
Tryptophan2953405-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Ultraviolet Fluorescence ApplicationsDescribes the benefits of UV excitation for fluorescence applications
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
UV ImagingAn overview of ultraviolet imaging applications, including solutions from Semrock and additional learning resources.



TRP-A Tryptophan Single-Band FF01-280/20 FF01-357/44 FF310-Di01 280/20 357/44 310 FF310 Filter Filters Set Sets TRP-A-000 TRP-A-L01 TRP-A-NQF TRP-A-NTE TRP-A-OMF TRP-A-OFF TRP-A-ZHE TRP-A-LDMP TRP-A-LDMK

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