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BrightLine Basic™ long-pass filter set, optimized for Calcofluor White and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: CFW-LP01-Clinical-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: CFW-LP01-Clinical

BrightLine Basic™ long-pass filter set, optimized for Calcofluor White and other like fluorophores

This filter set is optimized for the popular Calcofluor White fluorochrome used in a wide variety of mycological and fungal staining tests and kits. It also works well for DAPI and other blue fluorophores. For visual clinical applications, samples will exhibit amazingly low background and high contrast for easy and accurate identification in test after test. Compared to the CFW-BP01-Clinical bandpass filter set, the long-pass (LP01) set offers higher brightness and a dim "blue-white" background to provide clear color contrast (in addition to light-level contrast).

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details CFW-LP01-Clinical-000 (Standard size set, fits most microscopes2)$575In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmCFW-Ex01-Clin-25$305
Long Pass Emitter25 mm x 3.5 mmCFW-LP01-Clin-25$305
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmCFW-Di01-Clin-25x36$255
Click to expand/collapse details CFW-LP01-Clinical-L01 (Leica 'Large' set)$575Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details CFW-LP01-Clinical-NQF (set mounted in cube)$970In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details CFW-LP01-Clinical-NTE (set mounted in cube)$970In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details CFW-LP01-Clinical-OMF (set mounted in cube)$1,010In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details CFW-LP01-Clinical-OFF (set mounted in cube)$1,010In StockAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details CFW-LP01-Clinical-ZHE (set mounted in cube)$850In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details CFW-LP01-Clinical-LDMK (set mounted in cube)$925Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details CFW-LP01-Clinical-LDMP (set mounted in cube)$1,010In StockAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 5 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Alexa Fluor® 3503434413-Star
Alexa Fluor® 4054014221-Star
Alexa Fluor® 4304315411-Star
AMCA (Aminomethylcoumarin)3504481-Star
ATTO 3903924773-Star
BD Horizon V4504064493-Star
BD Horizon V5004105001-Star
BFP (EBFP)3804403-Star
Calcofluor White3514445-Star
Cascade Blue™4014191-Star
Cascade Yellow™3995493-Star
CellTrace calcein violet4004523-Star
CellTracker Violet BMQC+GSH4065263-Star
DiFMU-pH 9.03584501-Star
DyLight 4053984203-Star
ecliptic pHluorin pH5.54735071-Star
ER-Tracker™ Blue-White DPX3725571-Star
Fura Red™ (high pH)5726571-Star
Fura-2 (calcium free)3665151-Star
HiLyte Fluor™ 4054034281-Star
LIVE-DEAD Fixable Aqua Dead Cell Stain3675261-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034553-Star
Lucifer yellow4285441-Star
LysoSensor Blue3744243-Star
LysoTracker Blue3734221-Star
Marina Blue3634611-Star
Pacific Blue™4044553-Star
Pacific Orange4005513-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005431-Star
Qdot® 565 Nanocrystals3005641-Star
Qdot® 585 Nanocrystals3005881-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
Qdot® 655 Nanocrystals3006541-Star
Qdot® 705 Nanocrystals3007021-Star
SYPRO Ruby protein gel stain4696311-Star
ThiolTracker Violet+GSH4045263-Star
Vybrant DyeCycle Violet3694371-Star

Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Journal Article
Optical Filters for Clinical Fluorescence MicroscopyAtul Pradhan, PhD, Clinical Lab Products, January 2007
Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters
White Paper
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
Clinical MicroscopyAn overview of clinical microscopy, including solutions from Semrock and additional learning resources.

CFW-LP01-Clinical Calcofluor CalcofluorWhite Clinical Long-Pass-Filter-Set CFW-LP01-Clin CFW-Di01-Clin CFW-Ex01-Clin Filter Filters Set Sets 1,8-ANS Calcofluor-White Alexa-Fluor-350 ATTO-390 Cascade-Yellow CellTrace-calcein-violet CellTracker-Violet DAPI Dapoxyl DyLight-405 GFPuv LysoSensor-Blue mBBr+GSH Pacific-Blue Pacific-Orange sgBFP ThiolTracker-Violet-GSH CFW-LP01-Clinical-000 CFW-LP01-Clinical-L01 CFW-LP01-Clinical-NQF CFW-LP01-Clinical-NTE CFW-LP01-Clinical-OMF CFW-LP01-Clinical-OFF CFW-LP01-Clinical-ZHE CFW-LP01-Clinical-LDMP CFW-LP01-Clinical-LDMK

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