Welcome, Guest: Login or Register My Cart (0)
SetDetails
Item has been Discontinued. See Other Parts to Consider tab below.

BrightLine Basic™ long-pass filter set, optimized for Calcofluor White and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: CFW-LP01-Clinical-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 5 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig80-50
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
1,8-ANS3724773-Star
4-methylumbelliferone3574411-Star
7-hydroxy-4-methylcoumarin3604421-Star
Alexa Fluor® 3503434413-Star
Alexa Fluor® 4054014221-Star
Alexa Fluor® 4304315411-Star
AMCA (Aminomethylcoumarin)3504481-Star
ATTO 3903924773-Star
BD Horizon V4504064493-Star
BD Horizon V5004105001-Star
BFP (EBFP)3804403-Star
Calcofluor White3514445-Star
Cascade Blue™4014191-Star
Cascade Yellow™3995493-Star
CellTrace calcein violet4004523-Star
CellTracker Violet BMQC+GSH4065263-Star
ChlorophyllA4806501-Star
DAPI3594615-Star
Dapoxyl3735793-Star
DEAC4324721-Star
DiFMU-pH 9.03584501-Star
DY-4154184701-Star
DyLight 4053984203-Star
ECFP4354751-Star
ecliptic pHluorin pH5.54735071-Star
ER-Tracker™ Blue-White DPX3725571-Star
Fura Red™ (high pH)5726571-Star
Fura-2 (calcium free)3665151-Star
GFPuv3955093-Star
HiLyte Fluor™ 4054034281-Star
LIVE-DEAD Fixable Aqua Dead Cell Stain3675261-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034553-Star
Lucifer yellow4285441-Star
LysoSensor Blue3744243-Star
LysoTracker Blue3734221-Star
Marina Blue3634611-Star
mBBr+GSH3944903-Star
Pacific Blue™4044553-Star
Pacific Orange4005513-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005431-Star
Qdot® 565 Nanocrystals3005641-Star
Qdot® 585 Nanocrystals3005881-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
Qdot® 655 Nanocrystals3006541-Star
Qdot® 705 Nanocrystals3007021-Star
sgBFP™3874513-Star
Sirius3554241-Star
SpectrumBlue4054491-Star
SYPRO Ruby protein gel stain4696311-Star
TagBFP4024571-Star
ThiolTracker Violet+GSH4045263-Star
Vybrant DyeCycle Violet3694371-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Journal Article
TopicDescription
Optical Filters for Clinical Fluorescence MicroscopyAtul Pradhan, PhD, Clinical Lab Products, January 2007
Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
Clinical MicroscopyAn overview of clinical microscopy, including solutions from Semrock and additional learning resources.



CFW-LP01-Clinical Calcofluor CalcofluorWhite CFW-LP01-Clin CFW-Di01-Clin CFW-Ex01-Clin

The item has been added to your shopping cart.

The item has been added to your shopping cart, however, your IP address appears to be outside the US/ Canada, so online/ direct orders from Semrock are not allowed. Please see the International Distributor page to find your non-US distributor.

We apologize for any inconvenience.

The shopping cart is offline. Please call toll-free 1-866-SEMROCK (866-736-7625) or email us to place your order.