IDEX Health & Science continues to be the leader in spectrally complex optical filters and filter sets through its broad portfolio of Semrock Filters. In addition to optical filters, the IHS team also designs and manufactures complex optical assemblies, and LED and laser illumination systems that help enable technologies such as Next Generation Sequencing and Polymerase Chain Reaction (PCR), currently being used to identify and test for the SARS-CoV-2 virus.
Semrock offers a broad portfolio of optical filters suitable for singleplex and multiplex assays for real-time quantitative PCR (qPCR), reverse-transcription PCR (RT-PCR) and digital PCR (dPCR) fluorescence analysis for common fluorescent dyes including FAM, JOE, HEX, TAMRA, ROX and Cy5. In an effort to allow customers to better find these filters within our catalog product discovery, we've update the Individual Filters selection criteria to include PCR as an Application and each of these common fluorophores for the available bandpass filters suited for excitation and emission.
Here are quick links to product offerings related to the following fluorophores:
Determining the most suitable excitation and emission filters for your PCR system can be modeled within SearchLight. SearchLight is a dedicated website that allows fluorescence microscope users and optical instrument designers to evaluate the optimal spectral performance of fluorophores, filter sets, light sources, and detectors as components of an overall system. Will your existing filter set work with a new fluorophore or light source? What if a new excitation filter was installed or you changed cameras? With this tool, you can compare optical performance (signal or signal to background) when contemplating changes to any components in your system.
SearchLight allows you to upload your own spectra for any component and save and share results securely.
SearchLight can be found at: https://searchlight.semrock.com/. Use SearchLight now. Save time later.