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640 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for standard microscopy

Part Number: FF640-FDi01-25x36

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  • Your filter spectrum may differ slightly from the typical spectrum above, but is certified to meet the optical specifications noted below.
Semrock Optical Filter: FF640-FDi01  FF640  FDi01
640 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for standard microscopy
BrightLine® image-splitting dichroic beamsplitters offer superb image quality for both transmitted and reflected light when separating beams of light by color for simultaneous capture of multi-color images. For applications such as (FRET) and real-time live-cell imaging, users can now separate two, four or even more colors onto as many cameras or regions of a single camera sensor. The exceptional flatness of these filters virtually eliminates aberrations in the reflected beam for most common imaging systems. This dichroic beamsplitter is optimized for reflecting imaging beams up to 10 mm in diameter while minimizing RWE.

Common Fluorophore Pair to Split: Cy3/Cy5

Interested in an improved Flatness / RWE version? Consider Semrock's image-splitting dichoric beamsplitter for super-resolution microscopy:
λ/5 P-V RWE on 3mm FF640-FDi02-t3, optimized for reflecting imaging beams up to 37 mm in custom size versions.
Part NumberSizePrice1Stock StatusQuantity
FF640-FDi01-25x3625.2 mm x 35.6 mm x 1.1 mm
(unmounted)
$365In StockAdd To Cart

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Custom Sizing Limits:
Diameter Range: 5mm - 50.8mm
Rectangular Dimensions: 5mm - 55mm

Substrate thickness/tolerance as listed on Specifications tab.
Dimensional tolerances are ±0.1mm for unhoused parts.

For sizes outside the limits listed above, please contact customer service at 1-866-SEMROCK



1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
Reflection Band 1Ravg > 95% 350 – 629.5 nm
Edge Wavelength 1640 nm
Transmission Band 1Tavg > 93% 652 – 950 nm
Common Fluorophore Pair to SplitCy3/Cy5

General Filter Specifications
SpecificationValue
Angle of Incidence45 ± 1.5 degrees
Cone Half-angle2 degrees
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.
SteepnessStandard
Filter Effective Index1.74 Understanding ‘Effective Index of Refraction’ neff
Flatness / RWE ClassificationImage-splitting
Reflected Wavefront Error< 2λ P-V RWE @ 632.8 nm

Physical Filter Specifications (applies to standard sized parts; contact us regarding other sizes)
SpecificationValue
Transverse Dimensions (L x W)25.2 mm x 35.6 mm
Transverse Tolerance± 0.1 mm
Filter Thickness (unmounted)1.05 mm
Filter Thickness Tolerance (unmounted)± 0.05 mm
Clear Aperture≥ 80% (elliptical)
Scratch-Dig60-40
Substrate Thickness (unmounted)1.05 mm
Substrate Thickness Tolerance (unmounted)± 0.05 mm
OrientationReflective surface marked with part number - Orient in direction of incoming light

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity

640 Image-Splitting Imaging-Flat Dichroic Beamsplitter FF640 FDi FDi01 FF640-FDi01 Imaging Flat Single-edge Cy3/Cy5-Splitting FF640-FDi01-25x36

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