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580 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy

Part Number: FF580-FDi02-t3-25x36

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  • Your filter spectrum may differ slightly from the typical spectrum above, but is certified to meet the optical specifications noted below.
Semrock Optical Filter: FF580-FDi02  FF580  FDi02
580 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy
BrightLine® image-splitting dichroic beamsplitters offer superb image quality for both transmitted and reflected light when separating beams of light by color for simultaneous capture of multi-color images. For applications such as (FRET) and real-time live-cell imaging, users can now separate two, four or even more colors onto as many cameras or regions of a single camera sensor. The exceptional flatness of these filters virtually eliminates aberrations in the reflected beam for most common imaging systems.

Semrock's image-splitting dichroics for super-resolution microscopy deliver industry-leading λ/5 P-V RWE on 3 mm thick substrates for minimal focus shift and optical wavefront aberrations of the reflected imaging beam. These dichroics can be used with much larger diameter imaging beams up to 37 mm while minimizing RWE in custom size versions.

Common Fluorophore Pairs to Split: GFP/mCherry or FITC/TxRed

FF580-FDi01 image-splitting dichroic beamsplitter for standard micrscopy available on 1 mm substrate for reflecting imaging beams up to 10 mm in diameter while minimizing RWE
Part NumberSizePrice1Stock StatusQuantity
FF580-FDi02-t3-25x3625.2 mm x 35.6 mm x 3.0 mm
(unmounted)
$595In StockAdd To Cart

Don’t see a size you need? Create your own custom sized part – delivery confirmed ARO.

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Custom sized parts are non-returnable.
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Custom Sizing Limits:
Diameter Range: 5mm - 50.8mm
Rectangular Dimensions: 5mm - 55mm

Substrate thickness/tolerance as listed on Specifications tab.
Dimensional tolerances are ±0.1mm for unhoused parts.

For sizes outside the limits listed above, please contact customer service at 1-866-SEMROCK



1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
Reflection Band 1Ravg > 95% 350 – 570 nm
Edge Wavelength 1580 nm
Transmission Band 1Tavg > 93% 590.8 – 1200 nm
Common Fluorophore Pair to SplitGFP/mCherry or FITC/TxRed

General Filter Specifications
SpecificationValue
Angle of Incidence45 ± 1.5 degrees
Cone Half-angle2 degrees
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.
SteepnessStandard
Filter Effective Index1.84 Understanding ‘Effective Index of Refraction’ neff
Flatness / RWE ClassificationImage-splitting
Reflected Wavefront Error (3 mm thickness)< 0.2λ P-V RWE @ 632.8 nm

Physical Filter Specifications (applies to standard sized parts; contact us regarding other sizes)
SpecificationValue
Transverse Dimensions (L x W)25.2 mm x 35.6 mm
Transverse Tolerance± 0.1 mm
Filter Thickness (3 mm, unmounted)3.0 mm
Filter Thickness Tolerance (3 mm, unmounted)± 0.1 mm
Clear Aperture≥ 80% (elliptical)
Scratch-Dig60-40
Substrate Thickness (3 mm, unmounted)3.0 mm
Substrate Thickness Tolerance (3 mm, unmounted)± 0.1 mm
OrientationReflective surface marked with laser dot - Orient in direction of incoming light

3 mm Image-Splitting for Super-resolution Microscopy Wavelengths

FF484-FDi02.png

484 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy

• Imaging Dichroic Beamsplitter with edge at 484 nm
• Ravg > 95% 350 – 475 nm
• Tavg > 93% 492.3 – 1200 nm
• Common Fluorophore Pair to Split: DAPI/FITC
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF484-FDi02-t3-25x36
$595
(Non-US pricing info)



FF509-FDi02.png

509 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy

• Imaging Dichroic Beamsplitter with edge at 509 nm
• Ravg > 95% 350 – 500 nm
• Tavg > 93% 518.3 – 1200 nm
• Common Fluorophore Pair to Split: CFP/YFP
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF509-FDi02-t3-25x36
$595
(Non-US pricing info)



FF538-FDi02.png

538 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy

• Imaging Dichroic Beamsplitter with edge at 538 nm
• Ravg > 95% 350 – 528.4 nm
• Tavg > 93% 547.7 – 1200 nm
• Common Fluorophore Pair to Split: GFP/mOrange
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF538-FDi02-t3-25x36
$595
(Non-US pricing info)



FF560-FDi02.png

560 nm edge BrightLine® single-edgeimage-splitting dichroic beamsplitter for super-resolution microscopy

• Imaging Dichroic Beamsplitter with edge at 560 nm
• Ravg > 95% 350 – 550 nm
• Tavg > 93% 570.1 – 1200 nm
• Common Fluorophore Pair to Split: YFP/dTomato
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF560-FDi02-t3-25x36
$595
(Non-US pricing info)



FF580-FDi02.png

580 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy

• Imaging Dichroic Beamsplitter with edge at 580 nm
• Ravg > 95% 350 – 570 nm
• Tavg > 93% 590.8 – 1200 nm
• Common Fluorophore Pair to Split: GFP/mCherry
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF580-FDi02-t3-25x36
$595
(Non-US pricing info)



FF640-FDi02.png

640 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy

• Imaging Dichroic Beamsplitter with edge at 640 nm
• Ravg > 95% 350 – 629 nm
• Tavg > 93% 652 – 1200 nm
• Common Fluorophore Pair to Split: Cy3/Cy5
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF640-FDi02-t3-25x36
$595
(Non-US pricing info)



FF662-FDi02.png

662 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy

• Imaging Dichroic Beamsplitter with edge at 662 nm
• Ravg > 95% 350 – 650 nm
• Tavg > 93% 673.7 – 1200 nm
• Common Fluorophore Pair to Split: TxRed/Cy5
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF662-FDi02-t3-25x36
$595
(Non-US pricing info)


1 mm Image-Splitting for Standard Microscopy Wavelengths

FF484-FDi01.png

484 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for standard microscopy

• Imaging Dichroic Beamsplitter with edge at 484 nm
• Ravg > 95% 350 – 475 nm
• Tavg > 93% 492.3 – 950 nm
• Common Fluorophore Pair to Split: DAPI/FITC
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF484-FDi01-25x36
$365
(Non-US pricing info)



FF509-FDi01.png

509 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for standard microscopy

• Imaging Dichroic Beamsplitter with edge at 509 nm
• Ravg > 95% 350 – 500 nm
• Tavg > 93% 518.3 – 950 nm
• Common Fluorophore Pair to Split: CFP/YFP
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF509-FDi01-25x36
$365
(Non-US pricing info)



FF538-FDi01.png

538 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for standard microscopy

• Imaging Dichroic Beamsplitter with edge at 538 nm
• Ravg > 95% 350 – 528.4 nm
• Tavg > 93% 547.7 – 950 nm
•Common Fluorophore Pair to Split: GFP/mOrange
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF538-FDi01-25x36
$365
(Non-US pricing info)



FF560-FDi01.png

560 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for standard microscopy

• Imaging Dichroic Beamsplitter with edge at 560 nm
• Ravg > 95% 350 – 550 nm
• Tavg > 93% 570.1 – 950 nm
•Common Fluorophore Pair to Split: YFP/dTomato
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF560-FDi01-25x36
$365
(Non-US pricing info)



FF580-FDi01.png

580 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for standard microscopy

• Imaging Dichroic Beamsplitter with edge at 580 nm
• Ravg > 95% 350 – 570 nm
• Tavg > 93% 590.8 – 950 nm
• Common Fluorophore Pair to Split: GFP/mCherry
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF580-FDi01-25x36
$365
(Non-US pricing info)



FF640-FDi01.png

640 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for standard microscopy

• Imaging Dichroic Beamsplitter with edge at 640 nm
• Ravg > 95% 350 – 629.5 nm
• Tavg > 93% 652 – 950 nm
• Common Fluorophore Pair to Split: Cy3/Cy5
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF640-FDi01-25x36
$365
(Non-US pricing info)



FF662-FDi01.png

662 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for standard microscopy

• Imaging Dichroic Beamsplitter with edge at 662 nm
• Ravg > 95% 350 – 650 nm
• Tavg > 93% 673.7 – 950 nm
• Common Fluorophore Pair to Split: TxRed/Cy5
• Flatness / RWE: Image-splitting


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF662-FDi01-25x36
$365
(Non-US pricing info)


[x]
US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity

580 Image-Splitting Imaging-Flat Dichroic Beamsplitter FF580 FDi FDi02 FF580-FDi02 Imaging Flat Single-edge GFP/mCherry-Splitting FITC/TxRed-Splitting Super-Resolution P-V Flatness optical-wavefront-distortion reflected-wavefront-distortion Ultra-Flat RWE 1/5WaveRWE 1/5Wave 0.2WaveRWE 0.2Wave FF580-FDi02-t3-25x36

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