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421/491/572 nm BrightLine® triple-edge dichroic beamsplitter

Part Number: FF421/491/572-Di01-25x36

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  • Your filter spectrum may differ slightly from the typical spectrum above, but is certified to meet the optical specifications noted below.
Semrock Optical Filter: FF421/491/572-Di01  FF421/491/572  Di01
421/491/572 nm BrightLine® triple-edge dichroic beamsplitter
Multi-edge dichroics have two or more transitions from high reflectance to high transmission. Semrock offers a wide range of dichroic beamsplitters that exhibit steep edges with very high reflection and transmission bands. This standard epi-fluorescence dichroic beamsplitter is optimized only for broadband light sources such as LEDs and metal halide lamps.

BrightLine dichroic beamsplitter for DAPI/GFP/mCherry imaging channels optimized for Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm wavelengths.
Part NumberSizePrice1Stock StatusQuantity
FF421/491/572-Di01-25x36New Product25.2 mm x 35.6 mm x 1.1 mm
$545In StockAdd To Cart

Don’t see a size you need? Create your own custom sized part – delivery confirmed ARO.

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Diameter: mmDimension 1: mm
Dimension 2: mm 
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Custom Sizing Limits:
Diameter Range: 5mm - 50.8mm
Rectangular Dimensions: 5mm - 55mm

Substrate thickness/tolerance as listed on Specifications tab.
Dimensional tolerances are ±0.1mm for unhoused parts.

For sizes outside the limits listed above, please contact customer service at 1-866-SEMROCK

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
Reflection Band 1Ravg > 95% 350 – 400 nm
Reflection Band 2Ravg > 95% 400 – 413 nm
Edge Wavelength 1421 nm
Transmission Band 1Tavg > 93% 426 – 456 nm
Reflection Band 3Ravg > 95% 471 – 483 nm
Edge Wavelength 2491 nm
Transmission Band 2Tavg > 93% 498 – 524 nm
Reflection Band 4Ravg > 95% 541 – 560 nm
Edge Wavelength 3572 nm
Transmission Band 3Tavg > 93% 584 – 680 nm
Transmission Band 4Tavg > 93% 584 – 850 nm

General Filter Specifications
Angle of Incidence45 degrees (Tavg > 93%)
Angle of Incidence45 ± 1.5 degrees (Ravg > 95% & Tavg > 90%)
Cone Half-angle2 degrees
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.
Flatness / RWE ClassificationStandard Epi-fluorescence
Reflected Wavefront Error>> 6λ P-V RWE @ 632.8 nm
Filter Effective Index1.84 Understanding ‘Effective Index of Refraction’ neff

Physical Filter Specifications (applies to standard sized parts; contact us regarding other sizes)
Transverse Dimensions (L x W)25.2 mm x 35.6 mm
Transverse Tolerance± 0.1 mm
Filter Thickness (unmounted)1.05 mm
Filter Thickness Tolerance (unmounted)± 0.05 mm
Clear Aperture≥ 80% (elliptical)
Substrate Thickness (unmounted)1.05 mm
Substrate Thickness Tolerance (unmounted)± 0.05 mm
OrientationReflective surface marked with part number - Orient in direction of incoming light

Complementary Set Filters


441/511/717 nm BrightLine® triple-band bandpass filter

• Tavg > 90% 426 – 456 nm, Tavg > 90% 498 – 524 nm & Tavg > 90% 584 – 850 nm
• Center Wavelengths of 441 nm, 511 nm & 717 nm and GMBWs of 30 nm, 26 nm & 266 nm

Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF01-441/511/717-25
(Non-US pricing info)

US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets

421/491/572 Dichroic beamsplitter FF421/491/572 FF421/491/572-Di01 Multi-edge Triple-edge Triple Lumencor Celesta CELESTA ZIVA Ziva FF421/491/572-Di01-25x36

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