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416/500/582/657 nm BrightLine® quad-edge dichroic beamsplitter

Part Number: FF416/500/582/657-Di01-25x36x1.5

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  • Your filter spectrum may differ slightly from the typical spectrum above, but is certified to meet the optical specifications noted below.
Optical Specifications
Reflection Band 1Ravg > 95% 400 – 410 nm
Edge Wavelength 1416 nm
Transmission Band 1Tavg > 90% 422 – 473 nm
Reflection Band 2Ravg > 95% 486 – 491 nm
Edge Wavelength 2500 nm
Transmission Band 2Tavg > 90% 506 – 545 nm
Reflection Band 3Ravg > 95% 560 – 570 nm
Edge Wavelength 3582 nm
Transmission Band 3Tavg > 90% 586 – 617 nm
Reflection Band 4Ravg > 95% 633 – 647 nm
Edge Wavelength 4657 nm
Transmission Band 4Tavg > 90% 666 – 750 nm

General Filter Specifications
Angle of Incidence45 ± 1.5 degrees
Cone Half-angle2 degrees
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.
Filter Effective Index1.82 Understanding ‘Effective Index of Refraction’ neff
Flatness / RWE ClassificationStandard Epi-fluorescence
Reflected Wavefront Error>> 6λ P-V RWE @ 632.8 nm

Physical Filter Specifications (applies to standard sized parts; contact us regarding other sizes)
Transverse Dimensions (L x W)25.2 mm x 35.6 mm
Transverse Tolerance± 0.1 mm
Filter Thickness (unmounted)1.5 mm
Filter Thickness Tolerance (unmounted)± 0.1 mm
Clear Aperture≥ 80% (elliptical)
Substrate Thickness (unmounted)1.5 mm
Substrate Thickness Tolerance (unmounted)± 0.1 mm
OrientationReflective surface marked with part number - Orient in direction of incoming light

Closest Alternate Filter


410/504/582/669 nm BrightLine® quad-edge standard epi-fluorescence dichroic beamsplitter

• Dichroic Beamsplitter with edges at 410 nm, 504 nm, 582 nm & 669 nm
• Ravg > 95% 381.5 – 392.5 nm, Ravg > 95% 475 – 495 nm, Ravg > 95% 547 – 572 nm & Ravg > 95% 643 – 656 nm
• Tavg > 90% 420 – 460 nm, Tavg > 90% 510 – 531 nm, Tavg > 90% 589.5 – 623.5 nm & Tavg > 90% 677 – 722 nm
• Flatness / RWE: Standard Epi-fluorescence

Custom sizing available in less than a week (sizing fee applies).
Part # FF410/504/582/669-Di01-25x36
(Non-US pricing info)

US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets

FF416/500/582/657-Di01 416/500/562/657

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