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407/494/576 nm BrightLine® triple-band bandpass filter

Part Number: FF01-407/494/576-21.8-D

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  • Your filter spectrum may differ slightly from the typical spectrum above, but is certified to meet the optical specifications noted below.
Semrock Optical Filter: FF01-407/494/576
407/494/576 nm BrightLine® triple-band bandpass filter
Individual multiband fluorescence bandpass filters that utilize Semrock's patented single-substrate construction. These filters have extremely high transmission, steep and well-defined edges, and outstanding blocking between the passbands. All thin-film, hard-coated construction for unsurpassed performance and reliability.
Part NumberSizePrice1Stock StatusQuantity
FF01-407/494/576-2525 mm x 5.0 mm$495In StockAdd To Cart
FF01-407/494/576-3232 mm x 5.0 mm$811Confirmed AROAdd To Cart
FF01-407/494/576-21.8-D21.8 mm x 2.0 mm
$495In StockAdd To Cart

Don’t see a size you need? Create your own custom sized part – delivery confirmed ARO.

Select Shape & Dimensions:
Diameter Unhoused / Unmounted
Rectangular Unhoused / Unmounted
Housed / Mounted
Diameter: mmDimension 1: mm
Dimension 2: mm 
Edge Blacken:
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Custom sized parts are non-returnable.
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Custom Sizing Limits:
Diameter Range: 5mm - 50.8mm
Rectangular Dimensions: 5mm - 50.8mm

Substrate thickness/tolerance as listed on Specifications tab.
Dimensional tolerances are ±0.1mm for unhoused parts.

For sizes outside the limits listed above, please contact customer service at 1-866-SEMROCK

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
Transmission Band 1Tavg > 80% 400 – 414 nm
Center Wavelength 1407 nm
Guaranteed Minimum Bandwidth 114 nm
FWHM Bandwidth 1 (nominal)18 nm
Transmission Band 2Tavg > 85% 484 – 504 nm
Center Wavelength 2494 nm
Guaranteed Minimum Bandwidth 220 nm
FWHM Bandwidth 2 (nominal)24 nm
Transmission Band 3Tavg > 85% 566 – 586 nm
Center Wavelength 3576 nm
Guaranteed Minimum Bandwidth 320 nm
FWHM Bandwidth 3 (nominal)25 nm
Blocking Band 1ODavg > 6 300 – 388 nm
Blocking Band 2ODavg > 6 424 – 472 nm
Blocking Band 3ODavg > 6 517 – 549 nm
Blocking Band 4ODavg > 6 602 – 725 nm
Blocking Band 5ODavg > 6 725 – 850 nm

General Filter Specifications
Angle of Incidence0 ± 5 degrees
Cone Half-angle7 degrees
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.
Filter Effective Index2.05 Understanding ‘Effective Index of Refraction’ neff

Physical Filter Specifications (applies to standard sized parts; contact us regarding other sizes)
Transverse Dimensions (Diameter)25 mm
Transverse Tolerance (mounted)+ 0.0 / - 0.1 mm
Filter Thickness (Mounted)5.0 mm
Filter Thickness Tolerance (Mounted)± 0.1 mm
Clear Aperture≥ 21 mm
Substrate Thickness (unmounted)2.0 mm
Substrate Thickness Tolerance (unmounted)± 0.1 mm
OrientationArrow on ring indicates preferred direction of propagation of light

Complementary Set Filters: Emitter & Dichroic


457/530/628 nm BrightLine® triple-band bandpass filter

• Tavg > 80% 446 – 468 nm, Tavg > 85% 520 – 540 nm & Tavg > 85% 614 – 642 nm
• Center Wavelengths of 457 nm, 530 nm & 628 nm and GMBWs of 22 nm, 20 nm & 28 nm

Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF01-457/530/628-25
(Non-US pricing info)


436/514/604 nm BrightLine® triple-edge standard epi-fluorescence dichroic beamsplitter

• Dichroic Beamsplitter with edges at 436 nm, 514 nm & 604 nm
• Ravg > 97.5% 394 – 414 nm, Ravg > 97.5% 484 – 504 nm & Ravg > 97.5% 566 – 586 nm
• Tavg > 90% 446 – 468 nm, Tavg > 90% 520 – 540 nm & Tavg > 90% 614 – 642 nm
• Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF436/514/604-Di01-25x36
(Non-US pricing info)

Alternate # of Bands Multibandpass Filters
US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical DensityTables to describe the relationship between OD and transmission with tips for quickly calculating OD
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets

407/494/576 FF01-407/494/576 FF01 Bandpass Multibandpass Triple-Bandpass Triple-Band Triple FF01-407/494/576-25 FF01-407/494/576-32 FF01-407/494/576-21.8-D

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