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594 nm laser BrightLine® single-edge super-resolution / TIRF dichroic beamsplitter

Part Number: Di03-R594-t1-25x36

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  • Your filter spectrum may differ slightly from the typical spectrum above, but is certified to meet the optical specifications noted below.
Semrock Optical Filter: Di03-R594  Di03  R594
594 nm laser BrightLine® single-edge super-resolution / TIRF dichroic beamsplitter
The perfect dichroic beamsplitters for the most popular lasers used in fluorescence imaging, including all-solid-state lasers for advanced microscopy. All beamsplitters in this category have exceptional reflectance at the key laser wavelengths, and wider reflection bands — into UV for photoactivation and super-resolution techniques. Additionally they feature extended transmission regions — into IR to 1200 or 1600 nm, and anti-reflection (AR) coatings to minimize imaging artifacts resulting from the coherent laser light.

Semrock's super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and Super-resolution techniques such as TIRF, PALM, STORM, Structured Illumination, and STED.

1λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWE
λ/5 P-V RWE on 3 mm, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE
Part NumberSizePrice1Stock StatusQuantity
Di03-R594-t1-25x3625.2 mm x 35.6 mm x 1.1 mm
(unmounted)
$495In StockAdd To Cart
Di03-R594-t3-25x3625.2 mm x 35.6 mm x 3.0 mm
(unmounted)
$595In StockAdd To Cart

Don’t see a size you need? Create your own custom sized part – available in less than a week.

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Custom Sizing Limits:
Diameter Range: 5mm - 50.8mm
Rectangular Dimensions: 5mm - 55mm

Substrate thickness/tolerance as listed on Specifications tab.
Dimensional tolerances are ±0.1mm for unhoused parts.

For sizes outside the limits listed above, please contact customer service at 1-866-SEMROCK



1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
Reflection Band 1Rabs > 94% 593.5 – 594.3 nm
Reflection Band 1 (p-pol)Rabs > 90% 593.5 – 594.3 nm
Reflection Band 1 (s-pol)Rabs > 98% 593.5 – 594.3 nm
Reflection Band 2Ravg > 90% 350 – 593.5 nm
Edge Wavelength 1599.5 nm
Transmission Band 1Tavg > 93% 605 – 1200 nm
Laser Wavelengths 1593.5 nm, 594.1 nm, 594.0 +/- 0.3 nm

General Filter Specifications
SpecificationValue
Angle of Incidence45 degrees with a shift of 0.35%/degree (40 – 50 degrees)
Cone Half-angle0.5 degrees
Optical Damage Rating1 J/cm² @ 532 nm (10 ns pulse width)
SteepnessSteep
Filter Effective Index2.09 Understanding ‘Effective Index of Refraction’ neff
Flatness / RWE ClassificationSuper-resolution / TIRF
Reflected Wavefront Error (1 mm thickness)< 1λ P-V RWE @ 632.8 nm
Reflected Wavefront Error (3 mm thickness)< 0.2λ P-V RWE @ 632.8 nm

Physical Filter Specifications (applies to standard sized parts; contact us regarding other sizes)
SpecificationValue
Transverse Dimensions (L x W)25.2 mm x 35.6 mm
Transverse Tolerance± 0.1 mm
Filter Thickness (1 mm, unmounted)1.05 mm
Filter Thickness Tolerance (1 mm, unmounted)± 0.05 mm
Filter Thickness (3 mm, unmounted)3.0 mm
Filter Thickness Tolerance (3 mm, unmounted)± 0.1 mm
Clear Aperture≥ 80% (elliptical)
Scratch-Dig60-40
Substrate TypeFused Silica
Substrate Thickness (1 mm, unmounted)1.05 mm
Substrate Thickness Tolerance (1 mm, unmounted)± 0.05 mm
Substrate Thickness (3 mm, unmounted)3.0 mm
Substrate Thickness Tolerance (3 mm, unmounted)± 0.1 mm
OrientationReflective surface marked with laser dot - Orient in direction of incoming light

Complementary Set Filters

FF01-591_6.png

591/6 nm BrightLine® single-band bandpass filter

• Tavg > 93% 588 – 594.5 nm
• Center Wavelength of 591 nm and GMBW of 6 nm
• FWHM of 10.0 nm


Custom sizing available in less than a week (sizing fee applies).
Part # FF01-591/6-25
$405
(Non-US pricing info)



FF01-647_57.png

647/57 nm BrightLine® single-band bandpass filter

• Tavg > 92% 618.5 – 675.5 nm
• Center Wavelength of 647 nm and GMBW of 57 nm
• FWHM of 70 nm


Custom sizing available in less than a week (sizing fee applies).
Part # FF01-647/57-25
$325
(Non-US pricing info)


Edge Filters

BLP01-594R.png

594 nm EdgeBasic™ best-value long-pass edge filter

• Filter with 50% edge at 607 nm
• 411 cm-1 transition
• Tavg > 93% from 610.6 – 900 nm


Custom sizing available in less than a week (sizing fee applies).
Part # BLP01-594R-25
$375
(Non-US pricing info)


Notch Filters

NF03-594E.png

594 nm StopLine® single-notch filter

Laser Wavelength = 594.1 nm
• Typical Notch Bandwidth = 21.8 nm
• ODabs > 6 594.1 nm


Custom sizing available in less than a week (sizing fee applies).
Part # NF03-594E-25
$845
(Non-US pricing info)


[x]
US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Laser Damage ThresholdProvides information for calculating laser damage threshold for pulsed and continuous wave lasers
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Application
TopicDescription
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.

594 1/2 Wave 0.5Wave 1/2Wave 1/10 Wave 0.1Wave 1/10Wave Laser Flat Dichroic Beamsplitter Di03-R594 Di02-R594 R594 Laser-Flat Single-edge Di03-R Super-Resolution TIRF PALM STORM SIM Structured-Illumination structured-illumination-microscopy TIRF-illumination-systems STED P-V Flatness optical-wavefront-distortion reflected-wavefront-distortion Ultra-Flat 1WaveRWE RWE 1/5WaveRWE 1/5Wave 0.2WaveRWE 0.2Wave Di03-R594-t1-25x36 Di03-R594-t3-25x36

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