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488 nm laser BrightLine® single-edge super-resolution / TIRF dichroic beamsplitter

Part Number: Di03-R488-t1-25x36

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  • Your filter spectrum may differ slightly from the typical spectrum above, but is certified to meet the optical specifications noted below.
Semrock Optical Filter: Di03-R488  Di03  R488
488 nm laser BrightLine® single-edge super-resolution / TIRF dichroic beamsplitter
The perfect dichroic beamsplitters for the most popular lasers used in fluorescence imaging, including all-solid-state lasers for advanced microscopy. All beamsplitters in this category have exceptional reflectance at the key laser wavelengths, and wider reflection bands — into UV for photoactivation and super-resolution techniques. Additionally they feature extended transmission regions — into IR to 1200 or 1600 nm, and anti-reflection (AR) coatings to minimize imaging artifacts resulting from the coherent laser light.

Semrock's super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and Super-resolution techniques such as TIRF, PALM, STORM, Structured Illumination, and STED.

1λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWE
λ/5 P-V RWE on 3 mm, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE
Part NumberSizePrice1Stock StatusQuantity
Di03-R488-t1-25x3625.2 mm x 35.6 mm x 1.1 mm
(unmounted)
$495In StockAdd To Cart
Di03-R488-t3-25x3625.2 mm x 35.6 mm x 3.0 mm
(unmounted)
$595In StockAdd To Cart

Don’t see a size you need? Create your own custom sized part – delivery confirmed ARO.

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Custom Sizing Limits:
Diameter Range: 5mm - 50.8mm
Rectangular Dimensions: 5mm - 55mm

Substrate thickness/tolerance as listed on Specifications tab.
Dimensional tolerances are ±0.1mm for unhoused parts.

For sizes outside the limits listed above, please contact customer service at 1-866-SEMROCK



1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
Reflection Band 1Rabs > 94% 471 – 491 nm
Reflection Band 1 (p-pol)Rabs > 90% 471 – 491 nm
Reflection Band 1 (s-pol)Rabs > 98% 471 – 491 nm
Reflection Band 2Ravg > 90% 350 – 471 nm
Edge Wavelength 1495.5 nm
Transmission Band 1Tavg > 93% 499.8 – 1200 nm
Laser Wavelengths 1473 +/-2 nm & 488 +3/-2 nm

General Filter Specifications
SpecificationValue
Angle of Incidence45 degrees with a shift of 0.35%/degree (40 – 50 degrees)
Cone Half-angle0.5 degrees
Optical Damage Rating1 J/cm² @ 532 nm (10 ns pulse width)
SteepnessSteep
Filter Effective Index2.13 Understanding ‘Effective Index of Refraction’ neff
Flatness / RWE ClassificationSuper-resolution / TIRF
Reflected Wavefront Error (1 mm thickness)< 1λ P-V RWE @ 632.8 nm
Reflected Wavefront Error (3 mm thickness)< 0.2λ P-V RWE @ 632.8 nm

Physical Filter Specifications (applies to standard sized parts; contact us regarding other sizes)
SpecificationValue
Transverse Dimensions (L x W)25.2 mm x 35.6 mm
Transverse Tolerance± 0.1 mm
Filter Thickness (1 mm, unmounted)1.05 mm
Filter Thickness Tolerance (1 mm, unmounted)± 0.05 mm
Filter Thickness (3 mm, unmounted)3.0 mm
Filter Thickness Tolerance (3 mm, unmounted)± 0.1 mm
Clear Aperture≥ 80% (elliptical)
Scratch-Dig60-40
Substrate TypeFused Silica
Substrate Thickness (1 mm, unmounted)1.05 mm
Substrate Thickness Tolerance (1 mm, unmounted)± 0.05 mm
Substrate Thickness (3 mm, unmounted)3.0 mm
Substrate Thickness Tolerance (3 mm, unmounted)± 0.1 mm
OrientationReflective surface marked with laser dot - Orient in direction of incoming light

Complementary Set Filters

FF02-482_18.png

482/18 nm BrightLine® single-band bandpass filter

• Tavg > 93% 473 – 491 nm
• Center Wavelength of 482 nm and GMBW of 18 nm
• FWHM of 24.4 nm


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF02-482/18-25
$325
(Non-US pricing info)



FF01-525_45.png

525/45 nm BrightLine® single-band bandpass filter

• Tavg > 93% 502.5 – 547.5 nm
• Center Wavelength of 525 nm and GMBW of 45 nm
• FWHM of 50.6 nm


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # FF01-525/45-25
$325
(Non-US pricing info)


Laser Clean-up Filters

LL01-488.png

488 nm MaxLine® laser clean-up filter

• Laser Wavelength = 488 nm
• Tabs > 90% 488 nm
• FWHM of 1.9 nm (typical); 3.4 nm (maximum)


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # LL01-488-12.5
$325
(Non-US pricing info)



LD01-473_10.png

473/10 nm MaxDiode™ laser clean-up filter

• Laser Wavelength = 473 nm
• Tavg > 90% 468 – 478 nm
• Center Wavelength of 473 nm and GMBW of 10 nm


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # LD01-473/10-12.5
$285
(Non-US pricing info)


Edge Filters

BLP01-488R.png

488 nm EdgeBasic™ best-value long-pass edge filter

• Filter with 50% edge at 500nm
• 497 cm-1 transition
• Tavg > 93% from 504.7 – 900nm


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # BLP01-488R-25
$375
(Non-US pricing info)


Notch Filters

NF03-488E.png

488 nm StopLine® single-notch filter

• Laser Wavelength = 488 nm
• Typical Notch Bandwidth = 14 nm
• ODabs > 6 488 nm


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # NF03-488E-25
$845
(Non-US pricing info)


Dichroic Beamsplitters

LPD02-488RU.png

488 nm RazorEdge Dichroic™ laser beamsplitter

• Laser Wavelength = 488 nm
• 203 cm-1 transition
• Tavg > 93% 494.3 – 756.4 nm
• Flatness / RWE: Laser


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # LPD02-488RU-25
$615
(Non-US pricing info)



NFD01-488.png

488 nm StopLine® notch laser dichroic beamsplitter

• Laser Wavelength = 488 nm
• R > 98% 488 nm
• Tavg > 90% 350 – 465 nm & 523 – 1600 nm
• Flatness / RWE: Laser


Custom sizing delivery confirmed ARO (sizing fee applies).
Part # NFD01-488-25x36
$695
(Non-US pricing info)


[x]
US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Laser Damage ThresholdProvides information for calculating laser damage threshold for pulsed and continuous wave lasers
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Application
TopicDescription
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.

488 1/2 Wave 0.5Wave 1/2Wave 1/10 Wave 0.1Wave 1/10Wave Laser Flat Dichroic Beamsplitter Di03-R488 FDi03-R488 Di02-R488 R488 Laser-Flat Single-edge Di03-R Super-Resolution TIRF PALM STORM SIM Structured-Illumination structured-illumination-microscopy TIRF-illumination-systems STED P-V Flatness optical-wavefront-distortion reflected-wavefront-distortion Ultra-Flat 1WaveRWE RWE 1/5WaveRWE 1/5Wave 0.2WaveRWE 0.2Wave Di03-R488-t1-25x36 Di03-R488-t3-25x36

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