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635 nm laser BrightLine® single-edge laser dichroic beamsplitter

Part Number: Di02-R635-25x36

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  • Your filter spectrum may differ slightly from the typical spectrum above, but is certified to meet the optical specifications noted below.
Semrock Optical Filter: Di02-R635  Di02  R635
635 nm laser BrightLine® single-edge laser dichroic beamsplitter

The perfect dichroic beamsplitters for the most popular lasers used in fluorescence imaging, including newer all-solid-state lasers. All beamsplitters in this category have exceptional reflectance at the laser wavelengths, and anti-reflection (AR) coatings to minimize imaging artifacts resulting from the coherent laser light. This laser dichroic beamsplitter is optimized for reflecting laser beams up to 2.5 mm in diameter while minimizing RWE.

Interested in an improved Flatness / RWE version? Consider Semrock's Super-resolution / TIRF dichroics:
1λ P-V RWE on 1mm Di03-R635-t1 , optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWE
λ/5 P-V RWE on 3mm Di03-R635-t3, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE
Part NumberSizePrice1Stock StatusQuantity
Di02-R635-25x3625.2 mm x 35.6 mm x 1.1 mm
(unmounted)
$405In StockAdd To Cart
Di02-R635-22x2922.0 mm x 29.0 mm x 1.1 mm
(unmounted)
$405Ships in 2 daysAdd To Cart

Don’t see a size you need? Create your own custom sized part – available in less than a week.

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Custom Sizing Limits:
Diameter Range: 5mm - 50.8mm
Rectangular Dimensions: 5mm - 55mm

Substrate thickness/tolerance as listed on Specifications tab.
Dimensional tolerances are ±0.1mm for unhoused parts.

For sizes outside the limits listed above, please contact customer service at 1-866-SEMROCK



1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
Reflection Band 1Rabs > 94% 632.8 – 647.1 nm
Reflection Band 1 (p-pol)Rabs > 90% 632.8 – 647.1 nm
Reflection Band 1 (s-pol)Rabs > 98% 632.8 – 647.1 nm
Reflection Band 2Ravg > 90% 350.0 – 632.8 nm
Edge Wavelength 1655.8 nm
Transmission Band 1Tavg > 93% 658.8 – 1200 nm
Laser Wavelengths 1632.8 nm, 635 +7/-3 nm, 647.1 nm

General Filter Specifications
SpecificationValue
Angle of Incidence45 degrees with a shift of 0.35%/degree (40 – 50 degrees)
Cone Half-angle0.5 degrees
Optical Damage Rating1 J/cm² @ 532 nm (10 ns pulse width)
SteepnessSteep
Filter Effective Index1.74 Understanding ‘Effective Index of Refraction’ neff
Flatness / RWE ClassificationLaser
Reflected Wavefront Error< 6λ P-V RWE @ 632.8 nm

Physical Filter Specifications (applies to standard sized parts; contact us regarding other sizes)
SpecificationValue
Transverse Dimensions (L x W)25.2 mm x 35.6 mm
Transverse Tolerance± 0.1 mm
Filter Thickness (unmounted)1.05 mm
Filter Thickness Tolerance (unmounted)± 0.05 mm
Clear Aperture≥ 80% (elliptical)
Scratch-Dig60-40
Substrate TypeFused Silica
Substrate Thickness (unmounted)1.05 mm
Substrate Thickness Tolerance (unmounted)± 0.05 mm
OrientationReflective surface marked with part number - Orient in direction of incoming light

Complementary Set Filters

FF01-640_14.png

640/14 nm BrightLine® single-band bandpass filter

• Tavg > 93% 633 – 647 nm
• Center Wavelength of 640 nm and GMBW of 14 nm
• FWHM of 20.2 nm


Custom sizing available in less than a week (sizing fee applies).
Part # FF01-640/14-25
$355
(Non-US pricing info)



FF01-676_29.png

676/29 nm BrightLine® single-band bandpass filter

• Tavg > 90% 661.5 – 690.5 nm
• Center Wavelength of 676 nm and GMBW of 29 nm
• FWHM of 36.1 nm


Custom sizing available in less than a week (sizing fee applies).
Part # FF01-676/29-25
$305
(Non-US pricing info)


Laser Clean-up Filters

LL01-633.png

632.8 nm MaxLine® laser clean-up filter

• Laser Wavelength = 632.8 nm
• Tabs > 90% 632.8 nm
• FWHM of 2.4 nm (typical); 4.4 nm (maximum)


Custom sizing available in less than a week (sizing fee applies).
Part # LL01-633-12.5
$305
(Non-US pricing info)



LD01-640_8.png

640/8 nm MaxDiode™ laser clean-up filter

• Laser Wavelength = 640 nm
• Tavg > 90% 636.3 – 644.3 nm
• Center Wavelength of 640 nm and GMBW of 8 nm


Custom sizing available in less than a week (sizing fee applies).
Part # LD01-640/8-12.5
$265
(Non-US pricing info)


Edge Filters

BLP01-633R.png

632.8 nm EdgeBasic™ best-value long-pass edge filter

• Filter with 50% edge at 645 nm
• 385.4 cm-1 transition
• Tavg > 93% from 650.5 – 1200 nm


Custom sizing available in less than a week (sizing fee applies).
Part # BLP01-633R-25
$355
(Non-US pricing info)



BLP01-635R.png

635 nm EdgeBasic™ best-value long-pass edge filter

• Filter with 50% edge at 655 nm
• 380 cm-1 transition
• Tavg > 93% from 660 – 1200 nm


Custom sizing available in less than a week (sizing fee applies).
Part # BLP01-635R-25
$355
(Non-US pricing info)


Notch Filters

NF03-633E.png

633 nm StopLine® single-notch filter

• Laser Wavelength = 632.8 nm
• Typical Notch Bandwidth = 25 nm
• ODabs > 6 632.8 nm


Custom sizing available in less than a week (sizing fee applies).
Part # NF03-633E-25
$845
(Non-US pricing info)


Dichroic Beamsplitters

LPD02-633RU.png

632.8 nm RazorEdge Dichroic™ laser beamsplitter

• Laser Wavelength = 632.8 nm
• 156 cm-1 transition
• Tavg > 93% 641 – 980.8 nm
• Flatness / RWE: Laser


Custom sizing available in less than a week (sizing fee applies).
Part # LPD02-633RU-25
$575
(Non-US pricing info)



NFD01-633.png

633 nm StopLine® notch laser dichroic beamsplitter

• Laser Wavelength = 632.8 nm
• R > 98% 632.8 nm
• Tavg > 90% 350 – 603 nm & 678 – 1600 nm
• Flatness / RWE: Laser


Custom sizing available in less than a week (sizing fee applies).
Part # NFD01-633-25x36
$695
(Non-US pricing info)



Di03-R635.png

635 nm laser BrightLine® single-edge super-resolution / TIRF dichroic beamsplitter

• Laser Dichroic Beamsplitter with edge at 655.8 nm
• Rabs > 94% 632.8 – 647.1 nm
• Ravg > 90% 350 – 632.8 nm 
• Tavg > 93% 658.8 – 1200 nm
• Flatness / RWE: Super-resolution / TIRF


Custom sizing available in less than a week (sizing fee applies).
Part # Di03-R635-t1-25x36
$445
(Non-US pricing info)


[x]
US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Laser Damage ThresholdProvides information for calculating laser damage threshold for pulsed and continuous wave lasers
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Application
TopicDescription
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.

635 Laser Flat Dichroic Beamsplitter Di02-R635 R635 Laser-Flat Single-edge Di02-R Di02-R635-25x36 Di02-R635-22x29

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