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Vol. 3, Issue 1

In This Issue:

Editor's Note

Feature Interview

Feature Article

Tips & Techniques

Ask Our Experts

Links We Like

Work for Semrock

New at Semrock

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Nick George, Editor

Welcome to the first issue of our Wavelengths Newsletter in 2012. In this issue we have an interview with Dr. Ammasi Periasamy, Professor of Biology and Director of the W.M. Keck Center for Cellular Imaging at University of Virginia. Dr. Periasamy is well known for his expertise in molecular imaging, especially FRET Microscopy.

We also have some interesting reading on super-resolution imaging that provides an excellent overview on the how and why we now can exceed the Abbe diffraction limit of light microscopy.

As our first issue in 2012, I wanted to let you know that 2011 was our best year in the history of the company and thank you for your support. Semrock has a culture of constant improvement, and thanks to your feedback, we have implemented several initiatives that allow us to reach these new milestones. Enjoy and thanks again.

- Nick George, Director of Product Marketing

Semrock, Inc. on LinkedIn



Semrock's Prashant Prabhat talks to Dr. Ammasi Periasamy, Center Director, W.M. Keck Center for Cellular Imaging. Dr. Periasamy is an internationally recognized expert in advanced microscopy techniques, particularly in the area of imaging live cells using FRET.


Super-resolution microscopy

The latest incarnation of the modern fluorescence microscope has led to a paradigm shift. This wave is about breaking the diffraction limit first proposed in 1873 by Ernst Abbe. The implications of this development are profound.
This new technology, called super-resolution microscopy, allows for the visualization of cellular samples with a resolution similar to that of an electron microscope, yet it retains the advantages of an optical fluorescence microscope. This means it is possible to uniquely visualize desired molecular species in a cellular environment, even in three dimensions and now in live cells – all at a scale comparable to the spatial dimensions of the molecules under investigation. This article provides an overview of some of the key recent developments in super-resolution



What are you waiting for? We now have a short new video that walks you through where and how to use this nifty tool.

Need to check angle of incidence to be sure your filter will work? No problem. Model your filter data and play with your application specifications.

Semrock part numbers

Center wavelength or glass diameter? Which product family is this?

See the breakdown on the system if you are unsure. It's really not that complex, we promise.


Semrock Engineers

Semrock is currently searching for a Filter Design Engineer and a Thin Film Coating Engineer. These positions are based in Rochester, NY.

If you think you have what it takes to work with Semrock, please submit your resume to our Talent Acquisition Team.

VersaChrome Filters

We now have two families of VersaChrome tunable bandpass filters. The New Extended Overlap filters offer between 4-12 nm of additional overlap to allow for system variation such as AOI accuracy, and cone-half-angles. These filters have OD 6 blocking over the full tuning range 0-60°. The original VersaChrome filters still offer a tuning range of greater than 12% of the normal-incidence wavelength with only five filters to cover the full visible spectrum.

New Laser Dichroic Beamsplitters with the steepest edges for higher through-put and signal collection, they also offer wider reflection regions that push into the UV for photoactivation and super resolution techniques.

Chances are you have already spoken to one of these highly knowledgable people.

From L to R:
Mike Andrews
Chris Fridd

Alli Hill




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