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Wavelengths Newsletter
Wavelengths Cell Photo
Vol. 2, Issue 4

In This Issue:

Editor's Note

Feature Interview

Feature Article

Tips & Techniques

Ask an Expert

Links We Like

Upcoming Events

New at Semrock

Did you know?

Download a Catalog

Access the Semrock
White Paper Library

Nick George

Welcome to the Fourth Issue of our Wavelengths Newsletter in 2011. In this issue we are excited to continue our discussion on overcoming pixel shift and registration problems during multicolor fluorescence imaging.

We also have an interview with Dr. Eric Potma, Assoc. Professor at UC Irvine, who’s research interests include quantitative imaging of biopolymers using Nonlinear Optical Microscopy and also characterizing the nonlinear optical properties of nanostructures.

For smartphone and tablet users, we recently launched our mobile website: m.semrock.com. Currently, the site has all of our technical information available. Now you can easily read up on angle of incidence or cone half angle while enjoying a latte at the coffee shop!

We would like this newsletter to be a collaborative project with the microscope community at large. We invite and encourage you to send us your ideas, tips, questions, ideas for improving the newsletter, articles and anything else you think we should know or share. Enjoy.

- Nick George, Director of Product Marketing

Eric Potma

Raman wild man Eric Potma talks to Neil Anderson about the future of CARS, NLO and other imaging techniques.

Read on...

Standard alignment

Our microscopy expert, Prashant Prabhat, Ph D, continues his series on Pixelshift and why it works.


Need to be sure the AOI won't effect your filter selection? MyLight easily allows you to manipulate your filter and replot the performance in real time using theory data. Available on all single filter detail pages on our website.

More details on MyLight...

- Look for this button at the top of every filter graph.

This question comes up occasionally. The Rayleigh Range is the distance from the beam waist (smallest focused spot) to the point where the waist has increased by a factor of √2.  Images focused within one Rayleigh Range of the waist appear to be in focus.  When working with gaussian beams (often an excellent model for a laser beam), the depth of focus is quantified by the Rayleigh Range.  In order for images to remain in focus, reflecting filters (dichroic beamsplitters, for example) need to be flat enough to cause the reflected beam to shift less than one Rayleigh Range.

See webpage for more information on the different levels of flatness of our dichroics.


During ASCB in Denver, Tuesday, 12/6
"Laser-based Microscope Techniques" with
Turan Erdogan, PhD, Nick George, and Prashant Prabhat, PhD. 1:45 pm, Room 104

Looking for microscopy courses for 2012?
Most of these are still open for new registration.

Analytics and Quantitative Light Microscopy, May 9-18

Quantitative Fluorescence Microscopy, May 18-25

EMBO Practical Course in Advanced Optical Microscopy, March 21-31

Semrock supports the Advanced Microscopy and Digital Imaging Workshop, January 17-20

Robert Beeson

Robert Beeson was recently named Semrock's new General Manager, replacing Michael Cumbo, the new President of IDEX Corporation, Optics & Photonics platform.

Craig Hodgson, PhD, was also named the new Director of Research & Development at Semrock.


Our highly experienced team are the first ones you reach when you contact us.

From L to R
Scott Sandruck, Director -
4 years
Lori Markowski - 6 years
Linda Kohler - 2.5 years
Tracy Kuriatnyk - 1 year
Amy Pitoniak - 4.5 years

Semrock ISR Team


The Standard in Optical Filters

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