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BrightLine Basic™ single-band filter set, optimized for Cy3.5 and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: Cy3.5-A-Basic-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Semrock Filter Set: Cy3.5-A-Basic

BrightLine Basic™ single-band filter set, optimized for Cy3.5 and other like fluorophores

This filter set is ideal for use with Cy3.5. It is also ideal for some of the new flavors of monomeric red fluorescent proteins, including mStrawberry. This hard-coated filter set is the best value on the market, delivering substantially higher brightness and contrast than the comparable premium soft-coated filter sets.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details Cy3.5-A-Basic-000 (Standard size set, fits most microscopes2)$795Confirmed AROAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-565/24-25$300
Single Band Emitter25 mm x 3.5 mmFF01-620/52-25$300
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF585-Di01-25x36$272
Click to expand/collapse details Cy3.5-A-Basic-L01 (Leica 'Large' set)$795Confirmed AROAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details Cy3.5-A-Basic-NQF (set mounted in cube)$1,194Confirmed AROAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details Cy3.5-A-Basic-NTE (set mounted in cube)$1,194Confirmed AROAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details Cy3.5-A-Basic-OMF (set mounted in cube)$1,244Confirmed AROAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details Cy3.5-A-Basic-OFF (set mounted in cube)$1,244Confirmed AROAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details Cy3.5-A-Basic-ZHE (set mounted in cube)$1,084Confirmed AROAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6
Click to expand/collapse details Cy3.5-A-Basic-LDMK (set mounted in cube)$1,154Confirmed AROAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details Cy3.5-A-Basic-LDMP (set mounted in cube)$1,244Confirmed AROAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 5 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-ROX (carboxy-X-rhodamine)5786043-Star
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)5425681-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Alexa Fluor® 5685796033-Star
Alexa Fluor® 5945906181-Star
Amplex UltraRed peroxidation product (pH 7.5)5685811-Star
AsRed 25765921-Star
ATTO 5505535761-Star
ATTO 5655635923-Star
ATTO 5905946241-Star
ATTO 5946016241-Star
BODIPY TR-X phallacidin5906211-Star
Calcium Crimson5896091-Star
Calcium Orange5896091-Star
CellTrace BODIPY TR methyl ester5986251-Star
CellTracker Red CMTPX5866141-Star
DyLight 5945936181-Star
Ethidium bromide5186031-Star
Ethidium homodimer5276171-Star
FluoSpheres Crimson fluorescent microspheres6206461-Star
FluoSpheres Red fluorescent microspheres5776073-Star
HCS LipidTOX Red neutral lipid stain5826163-Star
HCS LipidTOX Red phospholipidosis5846081-Star
HiLyte Fluor™ 5945926161-Star
LIVE-DEAD Fixable Red Dead Cell Stain5956131-Star
LysoTracker Red5735923-Star
Magnesium Orange5505741-Star
Merocyanine 5405595791-Star
MitoTracker™ Orange5515751-Star
MitoTracker™ Red5785983-Star
Nile red-phospolipid5536373-Star
Orange 5525525741-Star
pHrodo™, succinimidyl ester5605871-Star
Propidium Iodide (PI)3056171-Star
Pro-Q Diamond5565831-Star
Qdot® 585 Nanocrystals3005883-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
Red 5805806033-Star
Rhodamine phalloidin5585751-Star
Rhodamine Red-X5735913-Star
SNARF (carboxy) 514 Excitation pH95766383-Star
SNARF-1 488nm (ph 6.0)5485861-Star
SNARF-1 488nm (ph 9.0)5766383-Star
SNARF-1 514nm (ph 6.0)5495873-Star
SNARF-1 514nm (ph 9.0)5766383-Star
Sulforhodamine 101-EtOH5785933-Star
Tetramethylrhodamine dextran5545821-Star
Texas Red dextran5926131-Star
Texas Red DHPE5846081-Star
Texas Red®5926141-Star
TRITC (Tetramethylrhodamine)5525781-Star
X-Rhod-1 Indicator5806013-Star

Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Journal Article
Optical Filters for Clinical Fluorescence MicroscopyAtul Pradhan, PhD, Clinical Lab Products, January 2007
Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters
White Paper
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
Clinical MicroscopyAn overview of clinical microscopy, including solutions from Semrock and additional learning resources.

Cy3.5-A-Basic Cy3.5 Basic 565/24 620/52 585 FF585-Di01 FF01-565/24 FF01-620/52 FF585 Filter Filters Set Sets Cy3.5-A-Basic-000 Cy3.5-A-Basic-L01 Cy3.5-A-Basic-NQF Cy3.5-A-Basic-NTE Cy3.5-A-Basic-OMF Cy3.5-A-Basic-OFF Cy3.5-A-Basic-ZHE Cy3.5-A-Basic-LDMP Cy3.5-A-Basic-LDMK

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