| |
 |
|
|
 GFP/RFP
FRET Filter Set |
|
|
|
| Optimized
for these fluorophores |
Green:
GFP, EGFP, FITC, Cy2™, Alexa Fluor 488
Red: mCherry,
mStrawberry, dTomato, DsRed, TRITC, Cy3™ |
|
|
| Actual
measured data from typical filters |
|
|
| This
four-filter set is specially designed to achieve high-performance
fast FRET (Fluorescence
Resonance Energy Transfer) imaging for green donor fluorescence
and orange/red acceptor fluorescence. FRET measurements using
wide-field microscopy are challenging – using the best
possible filters can make a significant difference. These
filter combinations provide excellent brightness and signal-to-noise
ratio while minimizing the undesirable donor-acceptor crosstalk.
In systems with dual (exciter-emitter) filter wheels, try
our GFP/DsRed-2X2M-B
dual-band Sedat set for FRET. |
|
|
| Filter |
Average
Transmission |
Center
Wavelength |
Bandwidth
* |
| Exciter |
>
93% |
472
nm |
30
nm |
| Emitter
1 |
>
93% |
520
nm |
35
nm |
| Emitter
2 |
>
93% |
607
nm |
36
nm |
| |
Average
Reflection |
Reflection
Band |
Transmission
Band |
| Dichroic |
>
98% |
442
- 488 nm |
502
- 730 nm |
|
| * Bandwidth
is the minimum width over which the average transmission
exceeds the specified passband transmission; the nominal
full-width-at-half-maximum (FWHM) is approximately the
Bandwidth + 1% of the Center Wavelength. |
|
|
|
|
Exciter
Dichroic
Emitter
1 
Emitter
2 
