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GFP/DsRed
Full Dual Band Filter Set |
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| Optimized
for these fluorophores |
Green: GFP,
rsGFP, FITC, Alexa Fluor 488
Red: DsRed,
TRITC, Cy3, Texas Red®, Alexa Fluor 568 & 594 |
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| Actual
measured data from typical filters |
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This
full-multiband set is perfectly optimized for imaging GFP
and DsRed with high brightness, extremely low crosstalk,
and superb signal-to-noise ratio. The set is also ideal
for samples dual-labeled with FITC and TRITC, or GFP and
Cy3, among other fluorophore combinations. For a "Pinkel" version
of this filter set (replaces the dual-band exciter with
two single-band exciters) see the GFP/DsRed-2X-A set.
For a "Sedat" version of this filter set (replaces
the dual-band exciter and emitter with two single-band
exciters and two single-band emitters) see the GFP/DsRed-2X2M-A set.
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| Filter |
Average
Transmission |
Center
Wavelengths |
Bandwidth
* |
| Exciter |
> 90%,
90% |
468,
553 nm |
34,
24 nm |
| Emitter |
> 90%,
90%
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512,
630 nm |
23,
91 nm |
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Average
Reflection |
Reflection
Bands |
Transmission
Bands |
| Dichroic |
> 95%
> 95% |
457
- 480 nm
542 - 565 nm |
500
- 529 nm
584 - 679 nm |
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| * Bandwidth
is the minimum width over which the average transmission
exceeds the specified passband transmission; the nominal
full-width-at-half-maximum (FWHM) is approximately the
Bandwidth + 1% of the Center Wavelength. |
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Exciter
Dichroic
Emitter
