Semrock

BFP/GFP FRET Filter Set

Optimized for these fluorophores

Blue: BFP, DAPI, Hoechst, Alexa Fluor 350
Green: GFP, EGFP, FITC, Cy2™, Alexa Fluor 488

Actual measured data from typical filters




Exciter	Dichroic	Emitter 1
ASCII Data	ASCII Data	ASCII Data
		Emitter 2
		ASCII Data

Set description

This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for blue donor fluorescence and green acceptor fluorescence. FRET measurements using wide-field microscopy are challenging – using the best possible filters can make a significant difference. These filter combinations provide excellent brightness and signal-to-noise ratio while minimizing the undesirable donor-acceptor crosstalk.

Filter	Average Transmission	Center Wavelength	Bandwidth *
Exciter	> 85%	387 nm	11 nm
Emitter 1	> 93%	447 nm	60 nm
Emitter 2	> 93%	520 nm	35 nm
	Average Reflection	Reflection Band	Transmission Band
Dichroic	> 98%	344 - 404 nm	415 - 570 nm

* Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.