BrightLine^® single-band filter set, optimized for mCherry and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: mCherry-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Other Products to Consider
Specifications
Fluorophore
Technical Info

Optical Specifications

Specification	Value
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications

Specification	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hours.

Physical Filter Specifications

Specification	Value
Scratch-Dig	60-40
Exciter/Emitter Orientation	Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation	"Reflective coating side" should face toward light source and sample.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
2-dodecylresorufin-lipid	582	595
5-carboxynapthofluorescein (pH 10)	555	615
5-ROX (carboxy-X-rhodamine)	578	604
7-AAD (7-aminoactinomycin D)	548	648
Alexa Fluor^® 568	579	603
Alexa Fluor^® 594	590	618
AsRed 2	576	592
ATTO 565	563	592
ATTO 590	594	624
ATTO 594	601	624
BOBO™-3	570	605
BODIPY TR-X phallacidin	590	621
Calcium Crimson	589	609
Carboxynaphthofluorescein	599	674
CellTrace BODIPY TR methyl ester	598	625
CellTracker Red CMTPX	586	614
ChlorophyllA	480	650
Cy3.5™	578	591
DDAO	648	657
DsRed	559	583
DsRed-Express	556	584
dTomato	556	582
DY-590	582	599
DyLight 594	593	618
Ethidium bromide	518	603
Ethidium homodimer	527	617
FluoSpheres Crimson fluorescent microspheres	620	646
FluoSpheres Red fluorescent microspheres	577	607
HcRed1	588	618
HCS LipidTOX Red neutral lipid stain	582	616
HCS LipidTOX Red phospholipidosis	584	608
HiLyte Fluor™ 594	592	616
KFP-Red	575	599
LIVE-DEAD Fixable Red Dead Cell Stain	595	613
LysoTracker Red	573	592
mApple	566	594
mCherry	587	610
MitoTracker™ Red	578	598
mKate2	588	633
mPlum	589	649
mRFP	555	583
mRFP1	584	700
mRuby	558	605
mStrawberry	574	596
mTangerine	568	585
Nile red-phospolipid	553	637
PerCP	490	677
pHrodo™, succinimidyl ester	560	587
Propidium Iodide (PI)	305	617
Qdot^® 605 Nanocrystals	300	602
Qdot^® 625 Nanocrystals	300	621
Qdot^® 655 Nanocrystals	300	654
ReAsH-CCPGCC	592	606
Red 580	580	603
Rhodamine Red-X	573	591
SNARF (carboxy) 514 Excitation pH9	576	638
SNARF-1 488nm (ph 6.0)	548	586
SNARF-1 488nm (ph 9.0)	576	638
SNARF-1 514nm (ph 6.0)	549	587
SNARF-1 514nm (ph 9.0)	576	638
SpectrumOrange	554	587
SpectrumRed	587	615
Sulforhodamine 101-EtOH	578	593
Tetramethylrhodamine dextran	554	582
Texas Red dextran	592	613
Texas Red DHPE	584	608
Texas Red^®	592	614
TurboFP635(Katushka)	591	638
X-Rhod-1 Indicator	580	601
YOYO-3	611	631

Tech Note

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Cube Assembly Instructions	PDF and video instruction for installing filters into popular filter cubes
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence Filters	Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a Microscope	A guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?	Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Fluorescence Imaging with Quantum Dot Nanocrystals	Describes how the Qdot nanocrystal structure is unique and the Semrock filter sets available for use with Qdots.

Journal Article

Topic	Description
Achieving the Best Alignment for Fluorescent Images	Turan Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005 Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image

White Paper

Topic	Description
Spectral Modeling in Fluorescence Microscopy	Discusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection

Application

Topic	Description
High Performance Microscopy	An overview of high performance microscopy, including solutions from Semrock and additional learning resources.

Revised Filter Set

BrightLine^® single-band filter set, optimized for mCherry and other like fluorophores

High brightness and contrast single-band filter set. Ideal for use with: mRFP1, mCherry, mApple

Part # mCherry-C-000
$825
(Non-US pricing info)

US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

mCherry-B

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Semrock manufactures hundreds of standard optical filters for immediate shipping: bandpass filters, dichroic beamsplitters, edge and notch filters, Raman spectroscopy filters, mirrors and fluorescence filters sets. For Custom optical filters, engineering design or system design support, please contact us to discuss your specific needs.

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BrightLine® single-band filter set, optimized for mCherry and other like fluorophores

Part Number: mCherry-B-000

BrightLine® single-band filter set, optimized for mCherry and other like fluorophores

BrightLine^® single-band filter set, optimized for mCherry and other like fluorophores

BrightLine^® single-band filter set, optimized for mCherry and other like fluorophores