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BrightLine^® single-band filter set, optimized for DAPI and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: DAPI-1160B-000

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Technical Info
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BrightLine^® single-band filter set, optimized for DAPI and other like fluorophores

Highest Contrast DAPI set on the market. The preferred DAPI set for most applications, this filter set is optimized for achieving exceptionally low noise (background) yet high brightness, resulting in unsurpassed contrast (signal-to-noise ratio). Images of typical cell samples with DAPI-stained nuclei exhibit more than twice the contrast of those made with the leading competitor's set. The carefully optimized narrow, red-shifted exciter filter minimizes fluorophore photobleaching and sample phototoxicity. What's more, unlike soft-coated filters and absorbing glass filters used for DAPI excitation, the unique hard-coated exciter does not "burn-out" under the intense UV illumination of modern fluorescence light sources.

This set is the unparalleled choice for most situations, especially those involving digital imaging. For specialized applications where brightness trumps contrast, use our DAPI-5060C set, which has the highest brightness on the market.

Individual filters and filter sets (no cube):

These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.

Part Number

Price¹

Stock Status

Quantity

DAPI-1160B-000 (Standard size set, fits most microscopes²)

$825

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285

DAPI-1160B-000-ZERO (Standard size -ZERO pixel set)

$924

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285
-ZERO set certification		-ZERO	$99

DAPI-1160B-L01-ZERO (Leica 'Large' -ZERO pixel set)

$924

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	21.8 mm x 2.0 mm	FF01-387/11-21.8-D	$345
Single Band Emitter	23.3 mm x 2.0 mm	FF02-447/60-23.3-D	$345
Single Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF409-Di03-22x29	$285
-ZERO set certification		-ZERO	$99

Filter Role	Filter Size	Part Number	Price¹
-ZERO set certification		-ZERO	$99

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	32 mm x 3.5 mm	FF02-447/60-32	$565
Single Band Dichroic	32 x 44 x 1.1 mm (corners cut for OXL cube)	FF409-Di03-32x44-XL	$432

DAPI-1160B-U02 (Olympus large-size (FX) set for U-M710I)

$1,370

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	32 mm x 5.0 mm	FF01-387/11-32	$565
Single Band Emitter	32 mm x 3.5 mm	FF02-447/60-32	$565
Single Band Dichroic	32 x 44 x 1.1 mm (corners cut for OFX cube)	FF409-Di03-32x44-FX	$447

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:

These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.

Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

DAPI-1160B-NQF (set mounted in cube)

$1,220

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285
Quadfluor cube		NQF	$395

DAPI-1160B-NQF-ZERO (-ZERO pixel set mounted in cube)

$1,319

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285
Quadfluor cube		NQF	$395
-ZERO set certification		-ZERO	$99

Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

DAPI-1160B-NTE (set mounted in cube)

$1,220

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285
TE 2000 cube		NTE	$395

DAPI-1160B-NTE-ZERO (-ZERO pixel set mounted in cube)

$1,319

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285
TE 2000 cube		NTE	$395
-ZERO set certification		-ZERO	$99

Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

DAPI-1160B-OMF (set mounted in cube)

$1,260

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285
U-MF2 cube		OMF	$435

DAPI-1160B-OMF-ZERO (-ZERO pixel set mounted in cube)

$1,359

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285
U-MF2 cube		OMF	$435
-ZERO set certification		-ZERO	$99

Olympus U-FF for microscopes: BX53, BX63

DAPI-1160B-OFF (set mounted in cube)

$1,260

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285
U-FF cube		OFF	$435

DAPI-1160B-OFF-ZERO (-ZERO pixel set mounted in cube)

$1,359

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285
U-FF cube		OFF	$435
-ZERO set certification		-ZERO	$99

Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes

DAPI-1160B-OFX (set mounted in cube)

$2,065

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	32 mm x 5.0 mm	FF01-387/11-32	$565
Single Band Emitter	32 mm x 3.5 mm	FF02-447/60-32	$565
Single Band Dichroic	32 x 44 x 1.1 mm (corners cut for OFX cube)	FF409-Di03-32x44-FX	$447
U-M710I cube		OFX	$695

Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

DAPI-1160B-ZHE (set mounted in cube)

$1,100

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285
FL Cube EC P+C cube		ZHE	$275

DAPI-1160B-ZHE-ZERO (-ZERO pixel set mounted in cube)

$1,199

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285
FL Cube EC P+C cube		ZHE	$275
-ZERO set certification		-ZERO	$99

Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6

DAPI-1160B-LDMK-ZERO (-ZERO pixel set mounted in cube)

$1,274

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	21.8 mm x 2.0 mm	FF01-387/11-21.8-D	$345
Single Band Emitter	23.3 mm x 2.0 mm	FF02-447/60-23.3-D	$345
Single Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF409-Di03-22x29	$285
DM-K cube		LDMK	$350
-ZERO set certification		-ZERO	$99

Leica DMi8 (P-Cube) for microscopes: DMi8

DAPI-1160B-LDMP-ZERO (-ZERO pixel set mounted in cube)

$1,359

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285
DMi8 (P-Cube) cube		LDMP	$435
-ZERO set certification		-ZERO	$99

Aperio AMF for microscopes: ScanScope FL

DAPI-1160B-AMF-ZERO (-ZERO pixel set mounted in cube)

$1,389

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-447/60-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409-Di03-25x36	$285
AMF cube		AMF	$465
-ZERO set certification		-ZERO	$99

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

Optical Specifications

Specification	Value
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications

Specification	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hours.

Physical Filter Specifications

Specification	Value
Scratch-Dig	60-40
Exciter/Emitter Orientation	Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation	"Reflective coating side" should face toward light source and sample.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
1,8-ANS	372	477
4-methylumbelliferone	357	441
7-hydroxy-4-methylcoumarin	360	442
Alexa Fluor^® 350	343	441
Alexa Fluor^® 405	401	422
AMCA (Aminomethylcoumarin)	350	448
ATTO 390	392	477
BD Horizon V450	406	449
BFP (EBFP)	380	440
Calcofluor White	351	444
Cascade Blue™	401	419
CellTrace calcein violet	400	452
DAPI	359	461
DEAC	432	472
DiFMU-pH 9.0	358	450
DY-415	418	470
DyLight 405	398	420
ECFP	435	475
HiLyte Fluor™ 405	403	428
Hoechst 33258	352	455
Hoechst 33342	352	455
Hoechst 34580	392	440
LIVE-DEAD Fixable Violet Dead Cell Stain	403	455
LysoSensor Blue	374	424
LysoTracker Blue	373	422
Marina Blue	363	461
mBBr+GSH	394	490
Pacific Blue™	404	455
sgBFP™	387	451
Sirius	355	424
SpectrumBlue	405	449
TagBFP	402	457
Vybrant DyeCycle Violet	369	437

Tech Note

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Cube Assembly Instructions	PDF and video instruction for installing filters into popular filter cubes
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence Filters	Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a Microscope	A guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?	Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity

Journal Article

Topic	Description
Achieving the Best Alignment for Fluorescent Images	Turan Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005 Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image

White Paper

Topic	Description
Spectral Modeling in Fluorescence Microscopy	Discusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection

Application

Topic	Description
High Performance Microscopy	An overview of high performance microscopy, including solutions from Semrock and additional learning resources.

Alternate Blue Fluorophore Single-Band Sets

BrightLine^® single-band filter set, optimized for DAPI and other like fluorophores

Highest-brightness "no burn-out" single-band filter set. Ideal for use with: DAPI, Alexa Fluor 350, AMCA , BFP , Hoechst 33258, Hoechst 33342, Hoechst 34580, LysoSensor Blue, Marina Blue, Pacific Blue, sgBFP

Part # DAPI-5060C-000
$825
(Non-US pricing info)

BrightLine^® long-pass filter set, optimized for DAPI and other like fluorophores

High contrast long-pass filter set. Ideal for use with: DAPI, Alexa Fluor 350, AMCA , BFP , Hoechst 33258, Hoechst 33342, Hoechst 34580, LysoSensor Blue, Marina Blue, Pacific Blue, sgBFP

Part # DAPI-11LP-A-000
$825
(Non-US pricing info)

BrightLine^® long-pass filter set, optimized for DAPI and other like fluorophores

High brightness long-pass filter set. Ideal for use with: DAPI, Alexa Fluor 350, AMCA , BFP , Hoechst 33258, Hoechst 33342, Hoechst 34580, LysoSensor Blue, Marina Blue, Pacific Blue, sgBFP, Fluoro-Gold™

Part # DAPI-50LP-A-000
$825
(Non-US pricing info)

Green Fluorophore Single-Band Sets

BrightLine^® single-band filter set, optimized for Green Fluorescent Protein and other like fluorophores

High brightness and contrast single-band filter set. Ideal for use with: GFP , Cy2, CyQuant Cell Proliferation Assay, DiO, EGFP, sgGFP , wtGFP , YOYO-1, YO-PRO-1

Part # GFP-3035D-000
$825
(Non-US pricing info)

BrightLine^® single-band filter set, optimized for FITC and other like fluorophores

High brightness and contrast single-band filter set. Ideal for use with: FITC , 5-FAM, Alexa Fluor 488, BODIPY, Calcein, Fluo-4, MitoTracker Green, Oregon Green, Rhodamine 110, rsGFP

Part # FITC-3540C-000
$825
(Non-US pricing info)

Orange Fluorophore Single-Band Sets

BrightLine^® single-band filter set, optimized for TRITC and other like fluorophores

High brightness and contrast single-band filter set. Ideal for use with: TRITC , Alexa Fluor 546, 5-TAMRA , Alexa Fluor 532, SpectrumOrange, BODIPY TMR-X, DiI, POPO-3, PO-PRO-3, Rhodamine

Part # TRITC-B-000
$825
(Non-US pricing info)

BrightLine^® single-band filter set, optimized for Cy3 and other like fluorophores

High brightness and contrast single-band filter set. Ideal for use with: Cy3, DsRed, Alexa Fluor 555, Calcium Orange, LysoTracker Yellow, MitoTracker Orange, Phycoerythrin, Rhodamine Phalloidin, 5-TAMRA

Part # Cy3-4040C-000
$825
(Non-US pricing info)

Red Fluorophore Single-Band Sets

BrightLine^® single-band filter set, optimized for Cy5 and other like fluorophores

High brightness and contrast single-band filter set. Ideal for use with: Cy5, SpectrumFRed - Lipophilic Tracer, SYTO Red Fluorescent Nucleic Acid Stains 60, 62, 63, TOTO-3, TO-PRO-3

Part # Cy5-4040C-000
$825
(Non-US pricing info)

US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

DAPI-1160B DAPI 387/11 447/60 409 Single-Band High Contrast High-Contrast FF01-387/11 FF02-447/60 FF409-Di03 FF409 Filter Filters Set Sets 1,8-ANS Alexa-Fluor-350 AF350 Alexa350 Alexa-Fluor-405 AF405 Alexa405 AMCA Aminomethylcoumarin ATTO-390 BD-Horizon-V450 BFP EBFP CellTrace-calcein-violet DyLight-405 Hoechst Hoechst-33258 Hoechst-33342 Hoechst-34580 LysoSensor-Blue Marina-Blue Pacific-Blue sgBFP TagBFP Vybrant-DyeCycle-Violet DAPI-1160B-000 DAPI-1160B-000-ZERO DAPI-1160B-L01-ZERO DAPI-1160B-U02 DAPI-1160B-NQF DAPI-1160B-NQF-ZERO DAPI-1160B-NTE DAPI-1160B-NTE-ZERO DAPI-1160B-OMF DAPI-1160B-OMF-ZERO DAPI-1160B-OFF DAPI-1160B-OFF-ZERO DAPI-1160B-OFX DAPI-1160B-ZHE DAPI-1160B-ZHE-ZERO DAPI-1160B-LDMP-ZERO DAPI-1160B-LDMK-ZERO DAPI-1160B-AMF-ZERO

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Semrock manufactures hundreds of standard optical filters for immediate shipping: bandpass filters, dichroic beamsplitters, edge and notch filters, Raman spectroscopy filters, mirrors and fluorescence filters sets. For Custom optical filters, engineering design or system design support, please contact us to discuss your specific needs.

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BrightLine® single-band filter set, optimized for DAPI and other like fluorophores

Part Number: DAPI-1160B-000

BrightLine® single-band filter set, optimized for DAPI and other like fluorophores

BrightLine® single-band filter set, optimized for DAPI and other like fluorophores

BrightLine® long-pass filter set, optimized for DAPI and other like fluorophores

BrightLine® long-pass filter set, optimized for DAPI and other like fluorophores

BrightLine® single-band filter set, optimized for Green Fluorescent Protein and other like fluorophores

BrightLine® single-band filter set, optimized for FITC and other like fluorophores

BrightLine® single-band filter set, optimized for TRITC and other like fluorophores

BrightLine® single-band filter set, optimized for Cy3 and other like fluorophores

BrightLine® single-band filter set, optimized for Cy5 and other like fluorophores

BrightLine^® single-band filter set, optimized for DAPI and other like fluorophores

BrightLine^® single-band filter set, optimized for DAPI and other like fluorophores

BrightLine^® single-band filter set, optimized for DAPI and other like fluorophores

BrightLine^® long-pass filter set, optimized for DAPI and other like fluorophores

BrightLine^® long-pass filter set, optimized for DAPI and other like fluorophores

BrightLine^® single-band filter set, optimized for Green Fluorescent Protein and other like fluorophores

BrightLine^® single-band filter set, optimized for FITC and other like fluorophores

BrightLine^® single-band filter set, optimized for TRITC and other like fluorophores

BrightLine^® single-band filter set, optimized for Cy3 and other like fluorophores

BrightLine^® single-band filter set, optimized for Cy5 and other like fluorophores