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Press Release
Semrock Introduces BrightLine® Fluorescence Filter Sets Specially Optimized for FRET
New filter sets provide superb brightness and contrast for FRET imaging, with the no-burn-out reliability of exclusively hard-dielectric optical coatings.

ROCHESTER, N.Y., February 28, 2007 –

Semrock, Inc., the leading manufacturer of high-performance optical filters for the Biotech and Analytical Instrumentation markets, today announced that it has extended its popular BrightLine series of high-performance fluorescence filters to include sets specifically optimized for key Fluorescence Resonance Energy Transfer (FRET) imaging applications. These new sets are designed for the most popular live-cell compatible FRET fluorophore pairs: blue-to-green, cyan-to-yellow, and green-to-red fluorescent proteins (including new monomeric red fluorescent proteins such as mCherry). And, the sets work well with a variety of other fluorophore donor-acceptor pairs. Each set consists of one excitation filter, one dichroic beamsplitter, and two emission filters, so they are ideally suited for the demanding motion-insensitive detection FRET requires in microscopes that are outfitted with an emission filter wheel or with a dual-camera detection module.

Fluorescence Resonance Energy Transfer (FRET) is a powerful technique for characterizing distance-dependent interactions on a molecular scale. It is one of the few tools available that is able to measure intermolecular and intramolecular distance interactions both in-vivo and in-vitro. “Because FRET measurements are so difficult, having the right optical filters can make all the difference in order to maximize the fidelity with which accurate ratiometric calculations can be made,” said Dr. Turan Erdogan, CTO of Semrock. “We have responded to popular demand by introducing the only high-performance, hard-coated sets specially optimized for FRET. This unique performance is achieved by combining the industry-leading high transmission, steep edges and deep blocking that all BrightLine research sets are known for, with careful designs resulting from sophisticated optical system modeling. The optional certified “ZERO pixel shift” performance that is readily available for the most demanding multi-color imaging applications is facilitated by the single-substrate construction that is the hallmark of all BrightLine filters.”

Like all of Semrock’s standard BrightLine fluorescence filter sets, these new FRET sets are available from stock. And every Semrock filter, without exception, is made with hard dielectric optical coatings for permanent, “no burn-out” performance.

About Semrock:
Semrock manufactures, in high volume, optical filters that set the standard in performance and reliability for the biotech and analytical instrumentation markets. Semrock’s patented and patent-pending products include the highly acclaimed BrightLine series of fluorescence filters for research and clinical microscopy, high-throughput screening, real-time PCR, and other fluorescence-based instrumentation; exceptional filters for Raman spectroscopy, and unique biomedical laser system optics. Semrock’s innovative products are made possible by combining modern ion-beam sputtering with Semrock’s proprietary volume manufacturing technology. Founded in September 2000, Semrock is based in Rochester, New York, a well-known center of optics, and has sales offices throughout the United States. For additional information about Semrock, its products, and its team of international distributors, visit the company website at www.semrock.com.

BrightLine is a registered trademark of Semrock, Inc.

Contact:
Kim Brosius
Marketing Coordinator
Semrock, Inc.
kbrosius@semrock.com
585-594-7005
Copyright © 2008 Semrock, Inc.