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BrightLine® Fluorescence Filters

Superior Spectra!

  Exciters and Emitters for FITC  

Dichroic Beamsplitters for FITC

 
   

 

  BrightLine excitation and emission filters utilize Semrock's unique and patented single-substrate construction, which avoids scattering, absorption, and reflection losses that occur with the traditional multi-substrate laminated filters based on soft coatings, which have adhesives in the optical path. When combined with Semrock's state-of-the-art ion-beam sputtering deposition technology, the result is the highest possible transmission of any fluorescence filters on the market. BrightLine filter spectra also exhibit the steepest possible edges, precise wavelength accuracy, and extreme blocking for optimal signal-to-noise ratio performance.   A unique design and manufacturing approach gives BrightLine dichroic beamsplitters the steepest edges (even for unpolarized light) and the flattest, highest reflection and transmission bands. More complete reflection and transmission mean less stray light that causes undesirable background and reduces signal-to-noise ratio. In contrast, conventional soft-coated beamsplitters compromise the performance of fluorescence microscopes and instruments by throwing away light unnecessarily and allowing undesired background light, resulting in poorer signal-to-noise ratio.  

The spectra in the graphs above are taken from publicly available information sources, and are not specially chosen to artificially distort the comparison.




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