TRITC-A

     Highest brightness and contrast, low bleedthrough, single-band filter set

Optimized for these fluorophores
TRITC (Tetramethylrhodamine), Alexa Fluor 546™, 5-TAMRA (5-carboxytetramethylrhodamine, high pH), Alexa Fluor 532™, SpectrumOrange, BODIPY TMR-X, DiI, POPO-3, PO-PRO-3, Rhodamine
Complete list of fluorophores for this filter set

Set description
This filter set is ideal for use with TRITC and a number of Rhodamine dyes, as well as other orange fluorophores. This improved set offers the high brightness that has made Semrock famous, and yet with its narrow exciter filter, it also provides a high signal-to-noise ratio and minimizes the bleedthrough in co-labeling applications that is associated with shorter-wavelength yellow and green fluorescent markers, like yellow fluorescent protein (YFP), green fluorescent protein (GFP), and FITC. Note that the narrow excitation filter efficiently captures the 546 nm emission line of mercury or metal-halide-mercury arc lamps.

Related sets to consider
Cy3.5-A-Basic
FilterPart NumberAverage TransmissionCenter WavelengthBandwidth*
Single-band ExciterFF01-543/22-25> 93%543 nm22 nm
Single-band EmitterFF01-593/40-25> 93%593 nm40 nm
  Average ReflectionReflection BandTransmission Band
Single-band DichroicFF562-Di02-25x36> 98%499 - 554.5 nm569.5 - 730 nm
*Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth +1% of the Center Wavelength.