FURA2-B

     4-filter single-band set (with 2 exciters)

Optimized for these fluorophores
Fura-2, LysoSensor Yellow/Blue (high/ low pH)
Complete list of fluorophores for this filter set

Set description
This four-filter BrightLine fluorescence set is specially designed to achieve high-performance fast ratiometric imaging of Ca2+ using Fura-2 as an indicator. The carefully optimized excitation filters provide superb balance of free and saturated Ca2+ signals, while minimizing the crosstalk and noise, resulting in the most accurate ratiometric measurements. The high signal throughput of the filter set permits reliable Fura-2 (Ca2+) measurements, even when using light sources of relatively limited ultraviolet output. Independent laboratories have measured this set to have 2-4X the free and saturated signals as compared with filters from the leading competitor.

FilterPart NumberAverage TransmissionCenter WavelengthBandwidth*
Single-band ExciterFF01-340/26-25> 75%340 nm26 nm
Single-band ExciterFF01-387/11-25> 90%387 nm11 nm
Single-band EmitterFF01-510/84-25> 93%510 nm84 nm
  Average ReflectionReflection BandTransmission Band
Single-band DichroicFF409-Di02-25x36> 98%344 - 404 nm415 - 570 nm
*Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth +1% of the Center Wavelength.