DAPI-1160A
Highest-contrast "no burn-out" single-band set for UV excitation
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DAPI, Alexa Fluor 350™, AMCA (Aminomethylcoumarin), BFP (Blue Fluorescent Protein), Hoechst 33258, Hoechst 33342, Hoechst 34580, LysoSensor Blue, Marina Blue, Pacific Blue™, sgBFP™
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Complete list of fluorophores for this filter set
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Highest Contrast DAPI set on the market. The preferred DAPI set for most applications, this filter set is optimized for achieving exceptionally low noise (background) yet high brightness, resulting in unsurpassed contrast (signal-to-noise ratio). Images of typical cell samples with DAPI-stained nuclei exhibit more than twice the contrast of those made with the leading competitor's set. The carefully optimized narrow, red-shifted exciter filter minimizes fluorophore photobleaching and sample phototoxicity. What's more, unlike soft-coated filters and absorbing glass filters used for DAPI excitation, the unique hard-coated exciter does not "burn-out" under the intense UV illumination of modern fluorescence light sources.
This set is the unparalleled choice for most situations, especially those involving digital imaging. For specialized applications where brightness trumps contrast, use our DAPI-5060B set, which has the highest brightness on the market.
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| Single-band Dichroic | FF409-Di02-25x36 | > 98% | 344 - 404 nm | 415 - 570 nm |
*Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth +1% of the Center Wavelength.