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BrightLine® Multiphoton Fluorescence Filters
  Common Specifications

Common specifications for Multiphoton filters:   All BrightLine fluorescence filters adhere to a rigorously defined set of specifications to ensure consistent high performance.
Property
Emitter
Dichroic
Comments
 Guaranteed Transmission  > 90%  > 90%  Averaged over any 50 nm (emitter) or 10 nm (dichroic) window
 within the passband
 Typical Transmission  > 95%  > 95%
 Dichroic Reflection
LWP 
  N/A  > 98%  Averaged over any 30 nm window within the reflection band
SWP 
  N/A  See details specific to FF670-SDi01 and FF720-SDi01
 Angle of Incidence  0° ± 5°  45° ± 1.5°  Range of angles over which optical specs are guaranteed
 for collimated light
 Cone Half Angle [1]  7°  2°  For uniformly distributed non-collimated light
 Autofluorescence  Ultra-low  Ultra-low  
 Transverse Dimensions   25.0 mm  25.2 x 35.6 mm  
 Transverse Tolerance
 + 0.0 / – 0.1 mm
  ± 0.1 mm  
 Clear Aperture  > 22 mm  > 80%  Area over which all optical specs are met
 Thickness  3.5 mm  1.05 mm  
 Thickness Tolerance
 ± 0.1 mm
  ± 0.05 mm  
 Edge Chipping
 N/A 
  < 0.1 mm  Measured from substrate edge
 Ring Housing Material  Aluminum,
 black-anodized		  N/A  Exciter and emitter only
 Surface Quality
 60-40 scratch-dig
  As per MIL-C-48497A
 Transmitted Wavefront Error
< l / 4 RMS at l = 633 nm
  Peak-to-valley error < 5 x RMS
 Coating Type  "Hard" ion-beam-sputtered  
 Blocking

 Emitter filters have exceptional blocking over the Ti:Sapphire laser range as needed to achieve superb
 signal-to-noise ratios even when using an extended-response PMT or CCD camera or other silicon-based
 detector; detailed specifications on the main Multiphoton filter page.

 Pulse Dispersion  LWP dichroic beamsplitters are suitable for use with 100 femtosecond gaussian laser pulses. For
 SWP dichroic beamsplitters, see BrightLine Multiphoton SWP Dichroic Beamsplitter Group Delay  Dispersion & Phase Shift Difference page
 Reliability and Durability  Ion-beam-sputtered, hard-coated technology with epoxy-free, single-substrate construction for unrivaled  filter life and no "burn-out" even when subjected to high optical intensities for a prolonged period of time.
 BrightLine filters are rigorously tested and proven to MIL-STD-810F and MIL-C-48497A environmental
 standards.
 Emitter Orientation  The emitter orientation does not affect its performance; therefore there is no arrow on the ring to denote
 a preferred orientation.
 Dichroic Orientation  "Reflective coating side" should face toward sample; see diagrams below.
 Microscope Compatibility  These filters fit most standard-sized microscope cubes from Nikon, Olympus, and Zeiss and may also be
 mounted in optical-bench mounts. Contact us for special filter sizes.
 [1] Filter performance is likely to remain satisfactory for Cone Half Angles as large as 10° for exciters and emitters, and 3° for dichroics.
Configuration of filters in a microscope:  Because BrightLine filters are so durable, you can easily populate your own cubes, sliders, filter wheels, and other optical-bench mounts without having to worry about damaging the filters. To obtain the most optimal performance from the filters, the dichroic beamsplitter should be oriented properly. The diagrams below demonstrate both a LWP and SWP dichroic configuration along with how to determine the "reflective coating side" of a dichroic filter.
Long-wave-pass (LWP) dichroic configuration
(FF665-Di01 and FF735-Di01)
A long-wave-pass dichroic transmits the excitation laser and reflects the fluorescence. The dichroic should be oriented such that the reflective coating side faces toward the detector and the sample. The emitter orientation does not affect its performance; therefore there is no arrow on the ring to denote a preferred orientation.
Short-wave-pass (SWP) dichroic configuration
(FF670-SDi01)
A short-wave-pass dichroic reflects the excitation laser and transmits the fluorescence. The dichroic should be oriented such that the reflective coating side faces toward the laser and the sample. The emitter orientation does not affect its performance; therefore there is no arrow on the ring to denote a preferred orientation.
Dichroic beamsplitter "reflective coating side"
    Marked Dichroics
  If the dichroic has a small linear mark, the reflective coating side is facing you when the mark is vertical and in the lower right corner.
     
    Unmarked Dichroics
When viewing the dichroic with the reflective coating side down, you can see a double-reflection of a bright object and the thickness of the filter at the far edge is apparent.
  When viewing the dichroic with the reflective coating side up, you can see a predominantly single reflection of a bright object and the thickness of the filter at the far edge is not visible.

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