| |
 |
|
| |
|
| Optimized
for these fluorophores |
|
|
| Actual
measured data from typical filters |
|
|
| This
filter set is optimized to be simultaneously the brightest
and the highest signal-to-noise ratio (contrast) filter set
available for measuring YFP and its variants – no more
compromising or exchanging filter sets for different YFP experiments.
For CFP-YFP FRET measurements,
consider our economical FRET combination set FRET-CFP/YFP-A,
or our high-performance "Sedat" filter set CFP/YFP-2X2M-A. |
|
|
| Filter |
Average
Transmission |
Center
Wavelength |
Bandwidth
* |
| Exciter |
>
93% |
500
nm |
24
nm |
| Emitter |
>
93% |
542
nm |
27
nm |
| |
Average
Reflection |
Reflection
Band |
Transmission
Band |
| Dichroic |
>
98% |
488
- 512 nm |
528
- 625 nm |
|
| * Bandwidth
is the minimum width over which the average transmission
exceeds the specified passband transmission; the nominal
full-width-at-half-maximum (FWHM) is approximately the
Bandwidth + 1% of the Center Wavelength. |
|
|
| Do
you need to separate CFP and YFP emission paths in a 2-detector
system? Use an FF506-Di02
dichroic beamsplitter. |
|
|
|
Exciter
Dichroic
Emitter
