Semrock

TRITC-A Filter Set

Optimized for these fluorophores

TRITC, Alexa Fluor 546, 5-TAMRA, Alexa Fluor 532, SpectrumOrange™, BODIPY TMR, DiI, POPO-3, PO-PRO-3, Rhodamine

Complete list of fluorophores for this filter set

Actual measured data from typical filters




Exciter	Dichroic	Emitter
ASCII Data	ASCII Data	ASCII Data

Set description

This filter set is ideal for use with TRITC and a number of Rhodamine dyes, as well as other orange fluorophores. This improved set offers the high brightness that has made Semrock famous, and yet with its narrow exciter filter, it also provides a high signal-to-noise ratio and minimizes the bleedthrough in co-labeling applications that is associated with shorter-wavelength yellow and green fluorescent markers, like yellow fluorescent protein (YFP), green fluorescent protein (GFP), and FITC. Note that the narrow excitation filter efficiently captures the 546 nm emission line of mercury or metal-halide-mercury arc lamps.

Filter	Average Transmission	Center Wavelength	Bandwidth *
Exciter	> 93%	543 nm	22 nm
Emitter	> 93%	593 nm	40 nm
	Average Reflection	Reflection Band	Transmission Band
Dichroic	> 98%	499 - 555 nm	569 - 730 nm

* Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.

Note: Historical limitations in filter technology led to the widespread use of TRITC emission filters that are overly broad and red-shifted, causing the TRITC fluorescence to appear red. Semrock’s new TRITC emission filter has been optimized for the best signal and noise performance, and since the TRITC emission band peaks in the orange the fluorescence will correctly look orange – not red. For those customers who prefer to forgo optimum performance for a more traditional red look (but still with quite acceptable performance) we recommend using our TRITC-A-Basic filter set.