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BrightLine® QD625-A Filter Set

   
QD625-A Filter Set
 Optimized for this fluorophore
Qdot® 625 Nanocrystals (from Invitrogen's Molecular Probes)
Actual measured data from typical filters
Exciter Dichroic Emitter
ASCII Data ASCII Data ASCII Data
 Set description

This single-band filter set is perfectly optimized for brilliant, dense multi-color detection with quantum dot nanocrystals. The highly transmitting, deep-blue exciter achieves maximum quantum dot excitation efficiency while virtually eliminating any DAPI or Hoechst excitation. The emitter has high transmission, steep edges, and a narrow bandwidth to perfectly match the Qdot 625 emission with minimal crosstalk from neighboring Qdots. With its exceptionally wide and flat transmission and reflection bands, the dichroic not only accommodates all Qdot nanocrystals but does so with a wide range of possible UV and blue excitation wavelengths. And with the "no burn-out" performance shared by all BrightLine filters, this set is the ideal tool for applications that depend on the superb photostability and archivability of quantum dots.

Filter Average Transmission Center Wavelength Bandwidth *
 Exciter > 90% 435 nm 40 nm
 Emitter > 90% 625 nm 15 nm
  Average Reflection Reflection Band Transmission Band
 Dichroic > 98% 327 - 488 nm 515 - 850 nm
* Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.
The next generation of superbright Qdot nanocrystals is here now! Invitrogen's new Qdot 625 is the brightest Qdot nanocrystal to date. Conjugated to streptavidin, it is ideal for labeling and detection of biotinlylated antibodies in immunostaining and western blot assays.
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