Semrock

QD525-A Filter Set

Optimized for this fluorophore

Qdot^® 525 Nanocrystals (from Invitrogen's Molecular Probes)

Actual measured data from typical filters




Exciter	Dichroic	Emitter
ASCII Data	ASCII Data	ASCII Data

Set description

This single-band filter set is perfectly optimized for brilliant, dense multi-color detection with quantum dot nanocrystals. The highly transmitting, deep-blue exciter achieves maximum quantum dot excitation efficiency while virtually eliminating any DAPI or Hoechst excitation. The emitter has high transmission, steep edges, and a narrow bandwidth to perfectly match the Qdot 525 emission with minimal crosstalk from longer-wavelength Qdots. With its exceptionally wide and flat transmission and reflection bands, the dichroic not only accommodates all Qdot nanocrystals but does so with a wide range of possible UV and blue excitation wavelengths. And with the "no burn-out" performance shared by all BrightLine filters, this set is the ideal tool for applications that depend on the superb photostability and archivability of quantum dots.

Filter	Average Transmission	Center Wavelength	Bandwidth *
Exciter	> 90%	435 nm	40 nm
Emitter	> 90%	525 nm	15 nm
	Average Reflection	Reflection Band	Transmission Band
Dichroic	> 98%	327 - 488 nm	515 - 850 nm

* Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.