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BrightLine® Fluorescence Filters

Microscopy Filters and Sets

Filter Sets Optimized for Fluorescence Microscopy
Semrock's BrightLine fluorescence filters are brighter, provide better contrast, and have been proven to be far more durable than conventional filters (no "burn-out" and you can even clean them with alcohol or acetone!). We stock a complete line of fluorescence filters and sets that are optimized for fluorescence microscopy applications.
  Single-band Filter Sets   Multiband Filter Sets   Other Microscopy Filter Products
 

• Highest Performance Sets

   • "Full Multiband" Sets • Multiphoton Filters
 

• Basic Series (Best Value)

  • Pinkel Sets • Individual Bandpass Filters
  • Laser Fluorescence Sets   • Sedat Sets • Individual Dichroic Beamsplitters
  • FISH Sets   • FISH Combination Sets   • Laser Dichroic Beamsplitter Filters
  • Quantum Dot Sets       • Individual Long Pass Laser Filters
  • FRET Sets   • Popular Instrument Filters
        • Microscope Filter Cubes
Highest-performance single-band filter sets:  We stock the following high-performance single-band fluorescence filters and sets that are fully optimized for fluorescence microscopy applications – for imaging one fluorophore at a time.
Color of
Emission
Filter Set Prefix
Compatible Fluorophores
Description
TRP-A  Unique UV Filter Set – Tryptophan, more …
 Ideal for imaging intrinsic fluorescence  from aromatic amino acids
DAPI-1160A  DAPI, Hoechst, AMCA, BFP, Alexa Fluor 350, more …
 Highest-contrast, preferred single-band  set for UV excitation
DAPI-5060B  DAPI, Hoechst, AMCA, BFP, Alexa Fluor 350, more …
 Highest-brightness single-band
 set for UV excitation
CFP-2432A  CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1, more …
 Highest brightness and contrast
 single-band filter set
FURA2-B  Fura-2 Ca2+ indicator, LysoSensor Yellow/Blue
 4-filter single-band set
 (with 2 exciters)
GFP-3035B  GFP, EGFP, DiO, Cy2™, YOYO-1, YO-PRO-1, more …
 High brightness and highest contrast
 single-band filter set
FITC-3540B  FITC, rsGFP, BODIPY, FAM, Fluo-4, Alexa Fluor 488, more …
 Highest brightness and contrast
 single-band filter set
YFP-2427A  YFP, Calcium Green-1, Eosin, Fluo-3, Rhodamine 123, more …
 Highest brightness and contrast
 single-band filter set
TRITC-A  Alexa Fluor 546, TRITC, Rhodamine, DiI, more …
 Highest brightness and contrast,
 low bleedthrough, single-band filter set
CY3-4040B  Cy3™, DsRed, PE, TAMRA, Alexa Fluor 532, more …
 Highest brightness and contrast
 single-band filter set
TXRED-4040B  Texas Red®, mCherry, 5-ROX, Alexa Fluor 568 & 594, more …
 Highest brightness and contrast
 single-band filter set
CY5-4040A  Cy5™, APC, Alexa Fluor 647 & 660, more …
 High brightness and contrast
 single-band filter set
CY5.5-A
 		 Cy5.5™, Alexa Fluor 680  High brightness and contrast
 single-band filter set
CY7-A
  Cy7™, Alexa Fluor 750  High brightness and contrast
 single-band filter set

NEW!  BrightLine Basic™ value-priced single-band filter sets:  These hard-coated single-band filter sets combine the proven “no burn-out” durability of Semrock's flagship highest-performance BrightLine research sets with optical performance that exceeds that of premium soft-coated fluorescence filters, yet are offered at soft-coated prices. In fact, hard-coated BrightLine Basic filter sets are brighter than soft-coated filter sets of comparable contrast, but don’t burn out, further lowering the total cost of ownership.
"Hard-coated performance at soft-coated prices™"
Compare BrightLine Basic filter sets to soft-coated filter sets and our highest-performance BrightLine filter sets...
Color of
Emission
Filter Set Prefix
Compatible Fluorophores
Description
 Calcofluor White, DAPI, Alexa Fluor 350  Long-pass filter set
 (for clinical testing)
 Calcofluor White, DAPI, Alexa Fluor 350  Best value single-band filter set
 BFP, DAPI, Hoechst, AMCA, Alexa Fluor 350  Best value single-band filter set
 CFP, Cerulean, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1  Best value single-band filter set
 wtGFP  Best value single-band filter set
 GFP, EGFP, DiO, Cy2™, YOYO-1, YO-PRO-1  Best value single-band filter set
 FITC, rsGFP, BODIPY, FAM, Fluo-4, Alexa Fluor 488  Best value single-band filter set
 FITC, Acridine Orange  Long-pass filter set
 (for clinical testing)
 YFP, EYFP, Calcium Green-1, Eosin, Fluo-3, Rhodamine 123  Best value single-band filter set
 TRITC, dTomato, Alexa Fluor 546, Rhodamine, DiI  Best value single-band filter set
 Cy3.5™, mStrawberry  Best value single-band filter set
 Texas Red®, mCherry, 5-ROX, Alexa Fluor 568 & 594  Best value single-band filter set

NEW!  BrightLine Laser fluorescence filter sets:  Crafted to take advantage of the superior resolution, higher sensitivity, and better image fidelity offered by today's state-of-the-art laser-based microscopes – including laser-scanning and spinning-disk confocal and TIRF microscopes. They are optimized for the most popular lasers used in fluorescence imaging, including newer all-solid-state lasers that are rapidly replacing older gas-laser technology. Find out more...
Color of
Emission
Filter Set Prefix
Compatible Fluorophores
Description
  DAPI, Hoechst, BFP, Alexa Fluor® 350 & 405, more …   Single-band filter set for laser
  microscopy
  GFP, FITC, Alexa Fluor® 488, EGFP, wtGFP, more …   Single-band filter set for laser
  microscopy
  RFP (mCherry, DsRed, ...), Texas Red, Cy3™, more …   Single-band filter set for laser
  microscopy
  Cy5™, Alexa Fluor® 633 and 647, APC, more ...   Single-band filter set for laser
  microscopy
NEW!  FISH single-band filter sets:  Semrock's"no-burn-out" FISH (Fluorescence In Situ Hybridization) fluorescence filters have been shown in independent clinical tests to as much as double your measurement speed! In stock for rapid delivery. Also see our new multiband combination sets!
Find out more...
How do BrightLine filters perform with densely spaced, multi-color assays? 

More about FISH and why BrightLine filter sets make FISH better...
Color of
Emission
Filter Set Prefix
Compatible Fluorophores
Description
DAPI-1160A  DAPI, Hoechst, Alexa Fluor® 350, more …
 Highest-contrast, preferred single-
 band set for UV excitation
SpAqua-A  SpectrumAqua™, DEAC
 Highest brightness and contrast
 single-band filter set
SpGr-A  SpectrumGreen™, FITC, Alexa Fluor® 488
 Highest brightness and contrast
 single-band filter set
SpGold-A  SpectrumGold™, Alexa Fluor® 546
 Highest brightness and contrast
 single-band filter set
SpOr-A  SpectrumOrange™, Cy3™, Rhodamine, Alexa Fluor® 555
 Highest brightness and contrast
 single-band filter set
SpRed-A  SpectrumRed™, Texas Red®, Alexa Fluor® 568 & 594
 Highest brightness and contrast
 single-band filter set
CY5-4040A  SpectrumFRed™ (Far Red), Cy5™, Alexa Fluor® 647 & 660
 High brightness and contrast
 single-band filter set
CY5.5-A  Cy5.5™, Alexa Fluor® 680  High brightness and contrast
 single-band filter set
CY7-A  Cy7™, Alexa Fluor® 750  High brightness and contrast
 single-band filter set
Qdot® single-band filter sets:  We stock these complete sets optimized for fluorescence applications based on Qdot® nanocrystals by Molecular Probes® (Invitrogen Detection Technologies), including the new Qdot 625 – the brightest Qdot to date.
Color of
Emission
Filter Set Prefix
Compatible Fluorophores
Description
QD525-A  Qdot 525 Nanocrystals  High brightness and contrast
 single-band filter set
QD605-A  Qdot 605 Nanocrystals  High brightness and contrast
 single-band filter set
QD625-A
  Qdot 625 Nanocrystals  High brightness and contrast
 single-band filter set
QD655-A  Qdot 655 Nanocrystals  High brightness and contrast
 single-band filter set
QDLP-A  Qdot 525, 565, 585, 605, 625, 655, 705, 800 Nanocrystals  Versatile and high brightness long-pass
 filter set for viewing multiple Qdots
FRET single-band filter sets:  We stock these complete sets optimized for Fluorescence Resonance Energy Transfer (FRET) imaging applications. Each set consists of one excitation filter, one dichroic beamsplitter, and two emission filters, so they are ideally suited for the demanding motion-insensitive detection FRET requires in microscopes that are outfitted with an emission filter wheel or with a dual-camera detection module. For more information on FRET, see our FRET technical note.
Color of
Emission
Filter Set Prefix
Compatible Fluorophores
Set Contents
FRET-BFP/GFP-A  Blue: BFP, DAPI, Hoechst, Alexa Fluor 350
 Green: GFP, EGFP, FITC, Cy2™, Alexa Fluor 488		  4-filter single-band set
 (with 2 emitters)
FRET-CFP/YFP-A  Cyan: CFP, CyPet, AmCyan
 Yellow: YFP, YPet, Venus		  4-filter single-band set
 (with 2 emitters)
FRET-GFP/RFP-A  Green: GFP, EGFP, FITC, Cy2™, Alexa Fluor 488
 Red: mCherry, mStrawberry, dTomato, DsRed, TRITC, Cy3™		  4-filter single-band set
 (with 2 emitters)
BrightLine ZERO™ Filter Sets – Semrock has uniquely solved the problem of poor image registration—now all you have to do is choose BrightLine ZERO fluorescence filter sets. All of the above BrightLine single-band filter sets are optionally available with certified "zero-pixel-shift" imaging performance. Composite images produced by conventional filter sets (left) are chromatically distorted, whereas BrightLine ZERO sets (right) yield precise multi-color images.
Multiband filter sets:  We stock the following high-performance multiband fluorescence filter sets – in "Full Multiband," "Pinkel" and "Sedat" configurations – for simultaneous and high-speed sequential imaging of samples with multiple fluorophores. For more information on the differences between these different set types, see the description of multiband filter set terminology.
 High-performance "Full Multiband" filter sets (with multiband exciter, dichroic, and emitter filters)
Color of
Emission
Filter Set Prefix
Compatible Fluorophores
Set Contents
CFP/YFP-A
 Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1   more …
 Yellow: YFP, Calcium Green-1, Eosin, Rhodamine 123
 Multiband set with dual-band  exciter, emitter, and  beamsplitter filters
GFP/DsRed-A
 Green: GFP, rsGFP, FITC, Alexa Fluor 488                      more …
 Red: DsRed, TRITC, Cy3, Texas Red®, Alexa Fluor 568 & 594
 Multiband set with dual-band  exciter, emitter, and  beamsplitter filters
FITC/TxRed-A

 Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor 488          more …
 Red: Texas Red®, mCherry, Alexa Fluor 568 & 594
 Multiband set with dual-band  exciter, emitter, and  beamsplitter filters
Cy3/Cy5-A
 Yellow:  Cy3™, DsRed, Alexa Fluor 555                          more …
 Red:  Cy5™, SpectrumFRed™, Alexa Fluor 647 & 660
 Multiband set with dual-band  exciter, emitter, and  beamsplitter filters
DA/FI/TX-B
 Blue: DAPI, Hoechst, AMCA, Alexa Fluor 350
 Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor 488          more …
 Red: Texas Red®, MitoTracker Red, Alexa Fluor 568 & 594
 Multiband set with triple-band  exciter, emitter, and  beamsplitter filters
 High-performance multiband "Pinkel" filter sets (with single-band exciter filters and multiband dichroic and emitter filters)
Color of
Emission
Filter Set Prefix
Compatible Fluorophores
Set Contents
CFP/YFP-2X-A
 Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1   more …
 Yellow: YFP, Calcium Green-1, Eosin, Rhodamine 123
 4-filter "Pinkel" dual-band set:
 2 exciters and dual-band emitter
 and beamsplitter filters
GFP/DsRed-2X-A
 Green: GFP, rsGFP, FITC, Alexa Fluor 488                      more …
 Red: DsRed, TRITC, Cy3, Texas Red®, Alexa Fluor 568 & 594
 4-filter "Pinkel" dual-band set:
 2 exciters and dual-band emitter
 and beamsplitter filters
GFP/HcRed-2X-A
 Green: GFP, rsGFP, FITC, Alexa Fluor 488                      more …
 Red: HcRed, Cy3.5, Texas Red®, Alexa Fluor 594
 4-filter "Pinkel" dual-band set:
 2 exciters and dual-band emitter
 and beamsplitter filters
FITC/TXRED-2X-A
Green:  FITC, GFP, rsGFP, Bodipy, Alexa Fluor 488          more …
Red:  Texas Red®, mCherry, Alexa Fluor 568 & 594
 4-filter "Pinkel" dual-band set:
 2 exciters and dual-band emitter
 and beamsplitter filters
CY3/CY5-2X-A
Yellow:  DsCy3, DsRed, Alexa Fluor 555                           more …
Red:  Cy5, SpectrumFRed, Alexa Fluor 647 & 660
 4-filter "Pinkel" dual-band set:
 2 exciters and dual-band emitter
 and beamsplitter filters
BFP/GFP/HcRed-
3X-A
 Blue: BFP, DAPI, Hoechst, AMCA, Alexa Fluor 350
 Green: GFP, rsGFP, FITC, Alexa Fluor 488                      more …
 Red: HcRed, Cy3.5, Texas Red®, Alexa Fluor 594
 5-filter "Pinkel" triple-band set:
 3 exciters and triple-band emitter
 and beamsplitter filters
CFP/YFP/HcRed-
3X-A
 Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1
 Yellow: YFP, Calcium Green-1, Fluo-3, Rhodamine 123     more …
 Red: HcRed, Cy3.5, Texas Red®, Alexa Fluor 594
 5-filter "Pinkel" triple-band set:
 3 exciters and triple-band emitter
 and beamsplitter filters
DA/FI/TX-3X-A
 Blue: DAPI, Hoechst, AMCA, Alexa Fluor 350
 Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor 488          more …
 Red: Texas Red®, MitoTracker Red, Alexa Fluor 568 & 594
 5-filter "Pinkel" triple-band set:
 3 exciters and triple-band
 emitter and beamsplitter filters
DA/FI/TR/Cy5-4X-A

 Blue: DAPI, Hoechst, AMCA, Alexa Fluor 350
 Green: FITC, GFP, rsGFP, BODIPY, Alexa Fluor 488        
 Orange: TRITC, Cy3, Texas Red®, MitoTracker Red,         more …
    Alexa Fluor 568 & 594
 Red: Cy5™, APC, TOTO-3, TO-PRO-3, Alexa Fluor 647 & 660
 6-filter “Pinkel” quad-band set:
 4 exciters and quad-band emitter
 and beamsplitter filters
 High-performance multiband "Sedat" filter sets (with single-band exciter and emitter filters and multiband dichroics)