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YFP-2427A Fluorescence Filter Set – Fluorophores

Table of fluorophores that may be used with the BrightLine® FF520 filter set:   The set is optimized for use with the fluorophores highlighted in bold. Note that results may vary depending upon instrumentation and conditions.
Fluorophore Peak Excitation
Wavelength* (nm)
Peak Emission
Wavelength* (nm)
 6-Carboxyrhodamine 6G 518 543
 BODIPY FL-Br2 531 545
 Calcium Green-1 506 531
 Calcium Green-2 (including Ca2+) 506 536
 Calcium Green-5N (including Ca2+) 506 532
 CI-NERF, CL-NERF 509 540
 DCFH (Dichlorodihydrofluorescein Diacetate) 505 535
 DHR (Dihydrorhodamine 123) 505 534
 DM-NERF 500 540
 Eosin 525 546
 Fluo-3 506 526
 JO-JO-1, JO-PRO-1 530 545
 Magnesium Green 506 531
 mHoneydew 478 561
 Rhodamine 123 507 529
 Rhodamine 6G 518 543
 Rhodamine Green 504 532
 SpectrumGreen 497 538
 SYTO Green Fluorescent Nucleic Acid Stains 11, 14, 15, 20, 22, 25 515 543
 SYTO Green Fluorescent Nucleic Acid Stains 80, 81, 82, 83 537 552
 TET 521 536
 TOTO-1, TO-PRO-1 509 533
 YFP 513 527
* These are typical wavelengths to be used for guidance purposes only. Actual peak wavelengths may vary for different applications.
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