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BrightLine® Laser Fluorescence Filters
Common Specifications

BrightLine fluorescence filters adhere to a rigorously defined set of specifications to ensure the highest possible performance repeated precisely in filter set after filter set.

Property
Value
Comment
 Reflection  > 98% (s-polarization)
 > 90% (p-polarization)
 > 94% (average polarization)
 Absolute reflectivity over the specified laser
 wavelengths/bands
 Transmission  > 93%  Averaged over the transmission passband
 Passband Wavelength Range  See table in BrightLine Laser Dichroics Page  
 Angle of Incidence  45.0°  Range for above optical specifications
 Based on a collimated beam of light
 Dependence of Wavelength on Angle of  Incidence (Edge Shift)  0.35% / degree  Linear relationship valid between about
 40° and 50°
 Cone Half Angle (for non-collimated light)  < 0.5°  Rays uniformly distributed and centered at 45°
 Substrate Material  Fused silica  Ultra-low autofluorescence
 Coating Type  "Hard" ion-beam sputtered  
 Clear Aperture  > 80%  Elliptical
 Size  25.2 mm x 35.6 mm (± 0.1 mm)  Unmounted
 Thickness  1.05 ± 0.05 mm  
 Transmitted Wavefront Error  < l / 4 RMS at l = 633 nm  Peak-to-valley error < 5 x RMS
 Beam Deviation  £ 10 arc seconds  
 Edge Chipping  < 0.1 mm  Measured from substrate edge
 Surface Quality  60-40 scratch-dig  As per MIL-C-48497A
 Second Surface  Anti-reflection (AR) coated
 Flatness  Reflection of a collimated, gaussian laser beam with waist diameter up to 2.5 mm causes
 less than one Rayleigh Range of focal shift after the objective or a focusing lens.
 Reliability and Durability  Ion-beam-sputtered, hard-coated technology with epoxy-free, single-substrate construction for
 unrivaled filter life and no "burn-out" even when subjected to high optical intensities for a
 prolonged period of time. BrightLine filters are rigorously tested and proven to MIL-STD-810F  and MIL-C-48497A environmental standards.
 Orientation  "Reflective coating side" should face toward light source and sample; see diagrams below.
 Microscope Compatibility  BrightLine filters are available to fit Leica, Nikon, Olympus, and Zeiss microscopes.
Orientation of filters in a microscope:   Because BrightLine filters are so durable, you can easily populate your own cubes, sliders, and filter wheels without having to worry about damaging the filters. To obtain the most optimal performance from the filters, they should be oriented properly. The diagrams below explain the proper orientation.
Proper orientation of all filters
 
The exciter and emitter should be oriented so that the arrow on the side of the aluminum ring points in the direction of propagation of the desired light – from the light source to dichroic for the exciter and from the dichroic to eye or camera for the emitter. The dichroic should be oriented such that the reflective coating side faces toward the exciter or light source and the sample.
Dichroic beamsplitter "reflective coating side"
Marked Dichroics
If the dichroic has a small linear mark, the reflective coating side is facing you when the mark is vertical (parallel to the long side) and in the lower right corner.
Unmarked Dichroics
  When viewing the dichroic with the reflective coating side down, you can see a double-reflection of a bright object and the thickness of the filter at the far edge is apparent.
  When viewing the dichroic with the reflective coating side up, you can see a predominantly single reflection of a bright object and the thickness of the filter at the far edge is not visible.

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