This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from DAPI and other similar fluorophores while offering superb blocking of popular 405 nm or 375 nm solid-state laser diodes. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.