Semrock

GFP-3035B Filter Set

Optimized for these fluorophores

GFP, Cy2™, CyQuant, DiO, EGFP, sgGFP™, wtGFP (non-UV excitation), YOYO-1, YO-PRO-1

Complete list of fluorophores for this filter set

Actual measured data from typical filters




Exciter	Dichroic	Emitter
ASCII Data	ASCII Data	ASCII Data

Set description

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) filter set available for measuring GFP and its variants—no more compromising or exchanging filter sets for different GFP experiments. For FRET analysis with GFP and another red fluorophore, like DsRed or mCherry, consider our economical filter set FRET-GFP/RFP-A, or our high-performance "Sedat" multiband filter set GFP/DsRed-2X2M-B. For applications that require a long-pass emission filter, consider our BrightLine Basic™ FITC-LP01-Clinical filter set.

Filter	Average Transmission	Center Wavelength	Bandwidth *
Exciter	> 93%	472 nm	30 nm
Emitter	> 93%	520 nm	35 nm
	Average Reflection	Reflection Band	Transmission Band
Dichroic	> 98%	442 - 488 nm	502 - 730 nm

* Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.

Do you need to separate GFP and RFP emission paths in a 2-detector system? Use an FF555-Di02 dichroic beamsplitter.