| |
 |
|
|
|
|
| Optimized
for these fluorophores |
| Fura-2 Ca2+ indicator,
LysoSensor Yellow/Blue |
|
|
| Actual
measured data from typical filters |
|
|
| This
four-filter BrightLine fluorescence set is specially designed
to achieve high-performance fast ratiometric imaging of
Ca2+ using Fura-2 as an indicator. The carefully
optimized excitation filters provide superb balance of
free and saturated Ca2+ signals, while minimizing
the crosstalk and noise, resulting in the most accurate
ratiometric measurements. The high signal throughput of
the filter set permits reliable Fura-2 (Ca2+)
measurements, even when using light sources of relatively
limited ultraviolet output. Independent laboratories have
measured this set to have 2-4X the free and saturated signals
as compared with filters from the leading competitor. |
|
|
| Filter |
Average
Transmission |
Center
Wavelength |
Bandwidth
* |
| Exciter
1 |
> 75% |
340
nm |
26
nm |
| Exciter
2 |
> 85% |
387
nm |
11
nm |
| Emitter |
> 93% |
510
nm |
84
nm |
| |
Average
Reflection |
Reflection
Band |
Transmission
Band |
| Dichroic |
> 98% |
344
- 404 nm |
415
- 570 nm |
|
| * Bandwidth
is the minimum width over which the average transmission
exceeds the specified passband transmission; the nominal
full-width-at-half-maximum (FWHM) is approximately the Bandwidth
+ 1% of the Center Wavelength. |
|
|
|
Exciter
1
Dichroic
Emitter
Exciter
2
