This
new "full-multiband" filter set allows for simultaneous
direct viewing or imaging with a color camera. Popular 2-fluorophore
combinations include FITC/Texas Red and GFP/mCherry. For a
"Pinkel" version of this filter set (replaces the
dual-band emitter with two single-band emitters) see the FITC/TXRED-2X-A
set, and for a "Sedat"
version of this set (replaces the dual-band emitter and exciter
with two single-band emitters and two single-band exciters)
see the FITC/TXRED-2X2M-A
set
Filter
Average
Transmission
Center
Wavelengths
Bandwidths
*
Exciter
>
90%, 90%
479,
585 nm
38,
27 nm
Emitter
>
90%, 90%
524,
628 nm
29,
33 nm
Average
Reflection
Reflection
Bands
Transmission
Bands
Dichroic
>
95%
> 95%
458
- 499 nm
570 - 600 nm
509
- 541 nm
612 - 647 nm
* Bandwidth
is the minimum width over which the average transmission
exceeds the specified passband transmission; the nominal
full-width-at-half-maximum (FWHM) is approximately the
Bandwidth + 1% of the Center Wavelength.