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FITC-3540B Fluorescence Filter Set – Fluorophores

Table of fluorophores that may be used with the BrightLine® FITC filter set:   The set is optimized for use with the fluorophores highlighted in bold. Note that results may vary depending upon instrumentation and conditions.
Fluorophore Peak Excitation
Wavelength* (nm)
Peak Emission
Wavelength* (nm)
 5-carboxy-2,7-dichlorofluorescein 504 529
 5-FAM (5-carboxyfluorescein) 492 518
 6-FAM 492 515
 Acridine Orange 500 526
 Acridine Orange + DNA 502 526
 Acridine Yellow 470 550
 Alexa Fluor 488 495 519
 Astrazon Orange R 470 540
 ATTO-TAG™ CBQCA 465 560
 Auramine 460 550
 BODIPY 505 513
 BODIPY 492/515 490 515
 BODIPY 505/515 502 510
 BTC 464 530
 Calcein 494 517
 CFDA 494 520
 CMFDA 494 520
 Cyclic AMP Fluorosensor (FiCRhR) 500 517
 DCFDA 504 529
 DTAF 494 520
 Emerald 490 510
 FAM 492 518
 FDA 494 520
 FITC (Fluorescein) 492 520
 Fluo-4 494 516
 Fluoro-Emerald 495 524
 FluorX 494 520
 JC-1 498 525
 JC-9 506 525
 LIVE/DEAD Kit Animal Cells 494 517
 LysoTracker Blue-White 466 536
 LysoTracker Green 504 511
 mHoneydew 478 561
 MitoTracker™ Green 490 516
 NBD 466 539
 NBD-X 467 538
 NeuroTrace 500/525 Green 497 524
 NeuroTrace 500/525 Green Fluorescent Nissl Stain 500 525
 Nissl 497 524
 Oregon Green™ 503 522
 Oregon Green™ 488 492 517
 Oregon Green™ 500 497 517
 Oregon Green™ 514 506 526
 PKH67 496 520
 Rhod-123 507 529
 Rhodamine 110 496 520
 Rhodol Green 496 523
 rsGFP (red shifted GFP, S65T) 498 516
 Sodium Green 506 532
 SYTO Green Fluorescent Nucleic Acid Stains 12, 13, 16, 21, 23, 24 494 515
 SYTOX Green 504 523
* These are typical wavelengths to be used for guidance purposes only. Actual peak wavelengths may vary for different applications.
 
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