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|
 |
| Optimized
for these fluorophores |
| FITC, FAM, Alexa Fluor 488, BODIPY, Calcein, Fluo-4, MitoTracker Green, Oregon Green, Rhodamine 110, rsGFP (red-shifted GFP, S65T) |
| Complete
list of fluorophores for this filter set |
|
|
| Actual
measured data from typical filters |
|
|
|
This
set is optimized for the most popular green fluorophores
like FITC (fluorescein isothiocyanate), FAM, and Alexa Fluor
488. It provides the highest brightness and signal-to-noise
ratio. It is also ideal for similar fluorophores that have
narrower, more closely spaced excitation and emission peaks,
like BODIPY. For a hard-coated yet economical FITC filter
set ideal for researchers on a tight budget, try
our FITC-A-Basic
set, or for a set optimized specifically for clinical applications
requiring a long-pass emission filter, see our FITC-LP01-Clinical
set.
|
|
|
| Filter |
Average
Transmission |
Center
Wavelength |
Bandwidth
* |
| Exciter |
> 93% |
482
nm |
35
nm |
| Emitter |
> 93% |
536
nm |
40
nm |
| |
Average
Reflection |
Reflection
Band |
Transmission
Band |
| Dichroic |
> 98% |
446
- 500 nm |
513
- 725 nm |
|
| * Bandwidth
is the minimum width over which the average transmission
exceeds the specified passband transmission; the nominal
full-width-at-half-maximum (FWHM) is approximately the Bandwidth
+ 1% of the Center Wavelength. |
|
|
| Do
you need a narrower exciter for reduced noise and photobleaching,
while maintaining a superb signal-to-noise ratio?
To create your own set, click on Prices
& Part Numbers for details of the standard dichroic
and emission filters from this set, and then select the narrower
FF01-494/20-25
exciter filter separately. |
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|
|
|
Exciter
Dichroic
Emitter
