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DAPI-5060B “High Brightness” Filter Set – Fluorophores

Table of fluorophores that may be used with the BrightLine® FF409 “High Brightness” filter set:   The set is optimized for use with the fluorophores highlighted in bold. Note that results may vary depending upon instrumentation and conditions.
Fluorophore Peak Excitation
Wavelength* (nm)
Peak Emission
Wavelength* (nm)
 1,8-ANS 372 480
 4-methylumbelliferone 360 440
 7-amino-4-methylcoumarin 351 430
 7-hydroxy-4-methylcoumarin 360 449
 ABQ 344 445
 Acridine 362 462
 Alexa Fluor 350™ 346 442
 AMCA (Aminomethylcoumarin) 345 425
 AMCA-X 353 442
 Aminocoumarin 350 445
 Anthrocyl stearate 370 446
 Beta lactamase 409 450
 BFP (Blue Fluorescent Protein) 382 448
 Bisbenzimide (Hoechst) 360 461
 Blancophor FFG 390 470
 Blancophor SV 370 435
 Calcein Blue 373 440
 Calcofluor White 395 440
 Cascade Blue™ 377 420
 Catecholamine 410 470
 Coumarin Phalloidin 387 470
 CPM Methylcoumarin 384 469
 DAPI 345 455
 EBFP 380 440
 Fast Blue 360 440
 FIF (Formaldehyde Induced Fluorescence) 405 433
 Gloxalic Acid 405 460
 Granular Blue 355 425
 Hoechst 33258 345 487
 Hoechst 33342 347 483
 Hoechst 34580 392 440
 HPTS 355 465
 Indo-1 346 475
 Intrawhite Cf  360 430
 Laurodan  355 460
 Leucophor PAF, SF, WS 370 430
 LysoSensor Blue 374 424
 LysoSensor Blue DND-167   374 425
 Lysosensor Blue DND-192 374 424
 LysoTracker Blue 373 422
 Mag-Indo-1 349 480
 Marina Blue 365 460
 Methoxycoumarin 360 410
 Monochlorobimane 380 461
 Pacific Blue™ 405 455
 PBFI 360 420
 Phorwite AR 360 430
 Phorwite BKL 370 430
 Phorwite Rev 380 430
 Phorwite RPA 375 430
 SBFI 360 420
 sgBFP™ 387 450
 SITS (Primuline) 410 450
 SITS (Stilbene Isothiosulphonic Acid) 365 460
 SPQ (6-methoxy-N-(3-sulfopropyl)quinolinium) 344 443
 Thioflavin TCN 350 460
 Thiolyte 378 483
 Tinopol CBS (Calcofluor White) 390 430
 True Blue 365 425
 Uvitex SFC 365 435
 Y66H 360 442
* These are typical wavelengths to be used for guidance purposes only. Actual peak wavelengths may vary for different applications.
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