Semrock

DAPI-5060B Filter Set – "Highest Brightness"

Optimized for these fluorophores

DAPI, Alexa Fluor 350, AMCA, BFP, Hoechst, LysoSensor Blue, Marina Blue, Pacific Blue, and sgBFP

Complete list of fluorophores for this filter set

Actual measured data from typical filters




Exciter	Dichroic	Emitter
ASCII Data	ASCII Data	ASCII Data

Set description

Brightest DAPI set on the market. This unique “flamethrower” filter set is recommended for those special cases in which it is critical to achieve the highest brightness for the shortest possible exposure times, or for very low fluorophore concentrations. For shorter exposure times this set exhibits up to 5 times more brightness but has lower contrast and more photobleaching than the DAPI-1160A set. For most applications that require low background, high contrast (signal-to-noise ratio), and minimized photobleaching, we recommend our DAPI-1160A set. In all cases our filters are uniquely hard-coated, for "no burn out" performance where it is most needed.

Filter	Average Transmission	Center Wavelength	Bandwidth *
Exciter	> 85%	377 nm	50 nm
Emitter	> 93%	447 nm	60 nm
	Average Reflection	Reflection Band	Transmission Band
Dichroic	> 98%	344 - 404 nm	415 - 570 nm

* Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.