Semrock

DAPI-1160A Filter Set – "Highest Contrast"

Optimized for these fluorophores

DAPI, Alexa Fluor 350, AMCA, BFP, Hoechst, LysoSensor Blue, Marina Blue, Pacific Blue, and sgBFP

Complete list of fluorophores for this filter set

Actual measured data from typical filters




Exciter	Dichroic	Emitter
ASCII Data	ASCII Data	ASCII Data

Set description

Highest Contrast DAPI set on the market. The preferred DAPI set for most applications, this filter set is optimized for achieving exceptionally low noise (background) yet high brightness, resulting in unsurpassed contrast (signal-to-noise ratio). Images of typical cell samples with DAPI-stained nuclei exhibit more than twice the contrast of those made with the leading competitor’s set. The carefully optimized narrow, red-shifted exciter filter minimizes fluorophore photobleaching and sample phototoxicity. What’s more, unlike soft-coated filters and absorbing glass filters used for DAPI excitation, the unique hard-coated exciter does not "burn out" under the intense UV illumination of modern fluorescence light sources.

This set is the unparalleled choice for most situations, especially those involving digital imaging. For specialized applications where brightness trumps contrast, use our DAPI-5060B set, which has the highest brightness on the market.

Filter	Average Transmission	Center Wavelength	Bandwidth *
Exciter	> 85%	387 nm	11 nm
Emitter	> 93%	447 nm	60 nm
	Average Reflection	Reflection Band	Transmission Band
Dichroic	> 98%	344 - 404 nm	415 - 570 nm

* Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.