Semrock

Cy3/Cy5-A Full Dual-band Filter Set

Optimized for these fluorophores

Yellow: Cy3™, DsRed, Alexa Fluor 555
Red: Cy5™, SpectrumFRed™, Alexa Fluor 647 & 660

Complete list of fluorophores for this filter set

Actual measured data from typical filters




Exciter	Dichroic	Emitter
ASCII Data	ASCII Data	ASCII Data

Set description

This new "full-multiband" filter set allows for simultaneous direct viewing or imaging with a color camera. For a "Pinkel" version of this filter set (replaces the dual-band emitter with two single-band emitters) see the Cy3/Cy5-2X-A set, and for a "Sedat" version of this set (replaces the dual-band emitter and exciter with two single-band emitters and two single-band exciters) see the Cy3/Cy5-2X2M-A set

Using a split-camera detection system and need to split your Cy3/Cy5 emission? Try our FF655-Di01-25x36.

Filter	Average Transmission	Center Wavelengths	Bandwidths *
Exciter	> 90%, 90%	534, 635 nm	36, 31 nm
Emitter	> 90%, 90%	577, 690 nm	24, 50 nm
	Average Reflection	Reflection Bands	Transmission Bands
Dichroic	> 95% > 95%	514 - 553 nm 617 - 652 nm	564 - 591 nm 665 - 718 nm

* Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.